Department of Biology (Zoology), University of Haripur, Haripur, Pakistan.
Department of Zoology, Shaheed Benazir Bhutto Women University Peshawar, Peshawar, Pakistan.
Reprod Domest Anim. 2022 Jul;57(7):798-801. doi: 10.1111/rda.14112. Epub 2022 Mar 25.
Present study aimed to evaluate field fertility rate and calf sex ratio of Nili Ravi buffalo semen sexed through modified swim-up method (Animal Reproduction Science, 182, 2017, 69). For this purpose, five mature Nili-Ravi buffalo bulls kept at semen production unit, Qadirabad, Pakistan, were selected. Two consecutive ejaculates per week were collected with artificial vaginą for 3 weeks. Qualified semen ejaculates were pooled and divided into two aliquots. The first aliquot was processed by routine procedure (control), whereas the second was processed by modified swim-up technique. After separation, semen was diluted in tris-citric acid extender and cryopreserved using standard techniques. Sexed semen was evaluated for fertility trials during peak breeding season. Artificially inseminated animals were examined for pregnancy rate through rectal palpation at least 3 months after insemination under field conditions. Calving ratio of female and male calves were recoded after Parturition. The fertility rate was higher (p < .05) in X-sorted sperm (70%) as compared with control (47%). The female calf ratio was higher (p < .05) in X-sorted sperm (78.58%) compared with control (53.3%). In Conclusion, conception rate and production of female calf were significantly higher with sexed semen separated through modified swim-up method compared with unsexed control.
本研究旨在评估通过改良泳动法分离的尼里-拉维水牛精液的现场受精率和犊牛性别比（动物繁殖科学，182，2017，69）。为此，选择了巴基斯坦卡迪拉巴德精液生产单位的 5 头成熟的尼里-拉维水牛公牛。每周采集两次连续的精液，使用人工阴道采集 3 周。合格的精液被收集并分成两份。第一份用常规程序处理（对照），第二份用改良泳动技术处理。分离后，精液用 tris-citric acid 稀释液稀释，并按照标准技术进行冷冻保存。在繁殖高峰期进行有性精液的受精试验。在人工授精后至少 3 个月，在野外条件下通过直肠检查检查怀孕率。分娩后记录雌性和雄性犊牛的出生比例。X 分选精子的受精率（70%）高于对照组（47%）（p < 0.05）。X 分选精子的雌性犊牛比例（78.58%）高于对照组（53.3%）（p < 0.05）。结论：与未分离的对照相比，通过改良泳动法分离的有性精液的受孕率和雌性犊牛的产仔率显著提高。
