Akhter S, Rakha B A, Ansari M S, Husna A U, Iqbal S, Khalid M
Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
Theriogenology. 2017 Jan 1;87:259-265. doi: 10.1016/j.theriogenology.2016.09.002. Epub 2016 Sep 9.
Egg yolk is used as a cryoprotectant for semen in different mammalian species including buffalo. Egg yolk from different sources may affect freezability of buffalo bull semen. Quail egg yolk (QEY) and turkey egg yolk (TEY) in Tris-citric acid extender was evaluated for post-thaw quality and in vivo fertility rate of cryopreserved buffalo bull semen. Ejaculates were collected on weekly basis from six Nili-Ravi buffalo bulls (12 ejaculates/bull) for a period of 6 weeks and diluted at 37 °C with tris-citric egg yolk extender (50 × 10 motile spermatozoa mL) containing different levels of QEY or TEY (5%, 10%, 15%, and 20%) or 20% chicken egg yolk (CEY; controls) and cryopreserved. Percent post-thaw sperm motility (48.3 ± 3.8), plasma membrane integrity (67.9 ± 5.3), live/dead ratio (68.2 ± 5.0), and viability (50.5 ± 3.7) were recorded higher (P < 0.05) in extender containing 5% QEY compared with control. However, TEY at 10% in extender improved (P < 0.05) the post-thaw sperm motility (57.5 ± 5.2), plasma membrane integrity (53.5 ± 4.5), livability (75.3 ± 6.0), and viability (73.5 ± 6.5) compared with higher concentrations of TEY and controls (20% CEY). The chromatin damage (2.0 ± 0.9) and intracellular enzymes, glutamic oxaloacetic transaminase (24.8 ± 3.5) and lactic dehydrogenase (77.7 ± 4.5), release were lower (P < 0.05) in extender containing 10% TEY compared with the controls. Invivo fertility was compared after artificial insemination with semen from two buffalo bulls that was cryopreserved in extenders containing 5% QEY, 10% TEY, or 20% CEY. A total of 600 inseminations (200 inseminations per extender) were recorded; the overall fertility rate was significantly higher (P < 0.05) with semen cryopreserved in extender containing 5% QEY (57.5 vs. 42%) and 10% TEY (57.5 vs. 42%). compared with 20% chiken egg yolk. In conclusion, QEY at 5% and TEY at 10% offers advantages over 20% CEY in terms of in vitro post-thaw semen quality and in vivo fertility of buffalo.
蛋黄被用作包括水牛在内的不同哺乳动物精液的冷冻保护剂。不同来源的蛋黄可能会影响水牛公牛精液的冷冻性能。对添加于柠檬酸三羟甲基氨基甲烷（Tris）缓冲液中的鹌鹑蛋黄（QEY）和火鸡蛋黄（TEY）在冷冻保存的水牛公牛精液解冻后的质量及体内受精率进行了评估。每周从6头尼里-拉菲水牛公牛收集射精精液（每头公牛12次射精），持续6周，并于37℃用含有不同水平QEY或TEY（5%、10%、15%和20%）或20%鸡蛋黄（CEY；对照）的柠檬酸三羟甲基氨基甲烷蛋黄缓冲液（50×10个活动精子/毫升）进行稀释，然后冷冻保存。与对照组相比，含5% QEY的缓冲液中解冻后精子活力百分比（48.3±3.8）、质膜完整性（67.9±5.3）、活/死比率（68.2±5.0）和生存力（50.5±3.7）更高（P<0.05）。然而，与更高浓度的TEY和对照组（20% CEY）相比，缓冲液中10%的TEY提高了（P<0.05）解冻后精子活力（57.5±5.2）、质膜完整性（53.5±4.5）、存活率（75.3±6.0）和生存力（73.5±6.5）。与对照组相比，含10% TEY的缓冲液中染色质损伤（2.0±0.9）以及细胞内酶谷草转氨酶（24.8±3.5）和乳酸脱氢酶（77.7±4.5）的释放更低（P<0.05）。用冷冻保存在含5% QEY、10% TEY或20% CEY缓冲液中的两头水牛公牛的精液进行人工授精后比较体内受精情况。共记录了600次授精（每种缓冲液200次授精）；与20%鸡蛋黄相比，冷冻保存在含5% QEY（57.5%对42%）和10% TEY（57.5%对42%）缓冲液中的精液总体受精率显著更高（P<0.05）。总之，就水牛精液解冻后的体外质量和体内受精而言，5%的QEY和10%的TEY比20%的CEY具有优势。
