Wen Ke, Li Xingang, Huang Rong, Nian Hai
The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, Guangdong 510642, People's Republic of China; The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, 510642 Guangdong, People's Republic of China; The National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, Guangdong 510642, People's Republic of China; The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou 510642, People's Republic of China; Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou 510642, People's Republic of China.
Ecotoxicol Environ Saf. 2022 Apr 1;234:113405. doi: 10.1016/j.ecoenv.2022.113405. Epub 2022 Mar 14.
Chromium is considered one of the most severe toxic elements affecting agriculture. Soybean seedlings under chromium stress were treated with glutathione and buthionine sulfoximine. The effects of exogenous glutathione on the physiological effects of two different chromium-resistant soybean seedlings and the expression levels of expression levels related genes were studied. This study tested the seedling weight and SPAD values, detected enzymatic antioxidants (i.e., superoxide dismutase, peroxidase, catalase, catalase, ascorbate peroxidase), and non-enzymatic antioxidants (i.e., glutathione, proline, soluble sugars, and soluble phenols) that attenuate chromium-induced reactive oxygen species, and quantified several genes associated with glutathione-mediated chromium stress. The results showed that exogenous glutathione could improve the physiological adaptability of soybean seedlings by regulating photosynthesis, antioxidant, and related enzyme activities, osmotic system, the compartmentalization of ion chelation, and regulating the transcription level of related genes, thereby increasing the chromium accumulation of soybean seedlings, enhancing the tolerance of chromium stress, and reducing the toxicity of chromium. Overall, the application of glutathione alleviates chromium toxicity in soybeans, and this strategy may be a potential farming option for soybean bioremediation in chromium-contaminated soils.
铬被认为是影响农业的最严重有毒元素之一。对铬胁迫下的大豆幼苗用谷胱甘肽和丁硫氨酸亚砜胺进行处理。研究了外源谷胱甘肽对两种不同耐铬大豆幼苗生理效应及相关基因表达水平的影响。本研究检测了幼苗重量和SPAD值，测定了减轻铬诱导的活性氧的酶促抗氧化剂（即超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶）和非酶促抗氧化剂（即谷胱甘肽、脯氨酸、可溶性糖和可溶性酚），并对与谷胱甘肽介导的铬胁迫相关的几个基因进行了定量分析。结果表明，外源谷胱甘肽可通过调节光合作用、抗氧化及相关酶活性、渗透系统、离子螯合的区室化以及调节相关基因的转录水平，提高大豆幼苗的生理适应性，从而增加大豆幼苗对铬的积累，增强对铬胁迫的耐受性，降低铬的毒性。总体而言，谷胱甘肽的应用减轻了大豆中的铬毒性，该策略可能是铬污染土壤中大豆生物修复的一种潜在耕作选择。
