Application of exogenous glutathione decreases chromium translocation and alleviates its toxicity in soybean (Glycine max L.).

Chromium is considered one of the most severe toxic elements affecting agriculture. Soybean seedlings under chromium stress were treated with glutathione and buthionine sulfoximine. The effects of exogenous glutathione on the physiological effects of two different chromium-resistant soybean seedlings and the expression levels of expression levels related genes were studied. This study tested the seedling weight and SPAD values, detected enzymatic antioxidants (i.e., superoxide dismutase, peroxidase, catalase, catalase, ascorbate peroxidase), and non-enzymatic antioxidants (i.e., glutathione, proline, soluble sugars, and soluble phenols) that attenuate chromium-induced reactive oxygen species, and quantified several genes associated with glutathione-mediated chromium stress. The results showed that exogenous glutathione could improve the physiological adaptability of soybean seedlings by regulating photosynthesis, antioxidant, and related enzyme activities, osmotic system, the compartmentalization of ion chelation, and regulating the transcription level of related genes, thereby increasing the chromium accumulation of soybean seedlings, enhancing the tolerance of chromium stress, and reducing the toxicity of chromium. Overall, the application of glutathione alleviates chromium toxicity in soybeans, and this strategy may be a potential farming option for soybean bioremediation in chromium-contaminated soils.