Zhou Qixing, Li Dandan, Zhang Suyan, Wang Simin, Hu Xiangang
Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education)/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Ecotoxicol Environ Saf. 2022 Apr 1;234:113420. doi: 10.1016/j.ecoenv.2022.113420. Epub 2022 Mar 14.
Endotoxin lipopolysaccharide (LPS) is a harmful substance commonly found in various environments that causes lung fibrosis. Exposure to PM also increases the risk of respiratory diseases. Through sulfur-carbon bonds and the edge S effect, GOQDs were used to bind in single-layer molybdenum disulfide (SLMoS) nanosheets to synthesize SLMoS@GOQDs heterojunction structures. GOQDs doping greatly increased the water solubility and stabilized of SLMoS. SLMoS@GOQDs with catalase-like activity protected cells from ultrastructural and cytomembrane damage and apoptosis induced by LPS. Moreover, the doping of GOQDs enhanced the escape of SLMoS@GOQDs from cellular uptake and suppressed the release of Mo ions. Nanosheet-cell interface interactions that were regulated by quantum dots supported these positive effects. Immunofluorescence analysis and cell imaging confirmed that the nanomaterial protected against cell injury by regulating the canonical Wnt/β-catenin pathway and the secretion of relevant cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Moreover, SLMoS@GOQDs also mitigated pneumonia caused by PM in vivo. Collectively, our findings not only provide a simple and effective approach to control lung diseases (caused by LPS or PM), but also reveal the potential value of heterojunction materials in the fields of toxicology and human health, boosting the application of nanotechnology in the fields of ecotoxicology and environmental safety.
内毒素脂多糖（LPS）是一种常见于各种环境中的有害物质，可导致肺纤维化。接触颗粒物（PM）也会增加患呼吸道疾病的风险。通过硫 - 碳键和边缘S效应，将石墨烯量子点（GOQDs）用于与单层二硫化钼（SLMoS）纳米片结合，以合成SLMoS@GOQDs异质结结构。GOQDs掺杂大大提高了SLMoS的水溶性和稳定性。具有过氧化氢酶样活性的SLMoS@GOQDs可保护细胞免受LPS诱导的超微结构和细胞膜损伤以及细胞凋亡。此外，GOQDs的掺杂增强了SLMoS@GOQDs从细胞摄取中的逃逸，并抑制了钼离子的释放。由量子点调节的纳米片 - 细胞界面相互作用支持了这些积极作用。免疫荧光分析和细胞成像证实，该纳米材料通过调节经典的Wnt/β-连环蛋白途径以及相关细胞因子如白细胞介素 - 6（IL - 6）和肿瘤坏死因子 - α（TNF - α）的分泌来预防细胞损伤。此外，SLMoS@GOQDs还可减轻体内由PM引起的肺炎。总的来说，我们的研究结果不仅提供了一种控制（由LPS或PM引起的）肺部疾病的简单有效方法，还揭示了异质结材料在毒理学和人类健康领域的潜在价值，推动了纳米技术在生态毒理学和环境安全领域的应用。
