Beloqui O, Nunes R M, Blades B, Berk P D, Potter B J
Alcohol Clin Exp Res. 1986 Aug;10(4):463-70. doi: 10.1111/j.1530-0277.1986.tb05125.x.
Incubation of freshly isolated rat hepatocytes with highly purified radiolabeled rat transferrin in weakly buffered medium in the presence of 10 mM ethanol resulted in a marked diminution of iron uptake by these cells, associated with a greater pH depression than in ethanol-free control studies. This effect on iron uptake persisted, even when the cells were preincubated for 90 min with ethanol before the addition of transferrin. Increasing the buffering capacity of the system or the addition of a metabolic inhibitor of alcohol dehydrogenase (4-methylpyrazole) returned iron uptake to control values. Acetaldehyde, acetate, lactate (products of ethanol metabolism), and 3-butanol (an alcohol not metabolized by alcohol dehydrogenase) had no influence on iron uptake. Further investigation of iron uptake over the pH range 6-8.5 revealed a marked dependency of iron uptake on the extracellular pH. Leucine incorporation into cell protein was also found to be pH dependent. It is suggested that, in the light of current understanding of transferrin recycling by other cell types, the disturbances of iron homeostasis observed in alcoholics can be partially accounted for by alterations in their acid-base metabolism.
在存在10 mM乙醇的弱缓冲培养基中，将新鲜分离的大鼠肝细胞与高度纯化的放射性标记大鼠转铁蛋白一起孵育，导致这些细胞对铁的摄取显著减少，与无乙醇对照研究相比，pH值下降更大。即使在添加转铁蛋白之前，细胞先用乙醇预孵育90分钟，这种对铁摄取的影响仍然存在。增加系统的缓冲能力或添加酒精脱氢酶的代谢抑制剂（4-甲基吡唑）可使铁摄取恢复到对照值。乙醛、乙酸、乳酸（乙醇代谢产物）和3-丁醇（一种不能被酒精脱氢酶代谢的醇）对铁摄取没有影响。在pH值6-8.5范围内对铁摄取的进一步研究表明，铁摄取明显依赖于细胞外pH值。还发现亮氨酸掺入细胞蛋白也依赖于pH值。根据目前对其他细胞类型转铁蛋白循环利用的理解，提示酗酒者中观察到的铁稳态紊乱可部分归因于其酸碱代谢的改变。
