Wallin B, Mørland J, Fikke A M
Acta Pharmacol Toxicol (Copenh). 1981 Aug;49(2):134-40. doi: 10.1111/j.1600-0773.1981.tb00882.x.
Rat liver parenchymal cells were isolated and incubated for 80 min. in buffered sale solutions. 14C-valine incorporation in the presence of a high concentration of unlabelled valine (4.2 mM) was taken as a measure of protein synthesis. The pH dependence of the synthesis of cell and medium proteins was studied at pH values varying from 6.6 to 7.9. The results showed that maximum protein synthesis occurred close to the physiological pH value. Protein synthesis was reduced at both lower and higher pH-values. Protein synthesis was inhibited by the addition of ethanol (30 mM). The relative inhibition caused by ethanol got more pronounced as the pH of the hepatocyte suspensions was lowered. The metabolism of ethanol resulted in lowering of the pH cell suspension. It is suggested that the depression of protein synthesis by ethanol could be mediated by the fall in pH due to ethanol metabolism.
分离大鼠肝脏实质细胞，并在缓冲盐溶液中孵育80分钟。在高浓度未标记缬氨酸（4.2 mM）存在的情况下，将14C-缬氨酸掺入作为蛋白质合成的指标。在pH值从6.6到7.9变化的条件下，研究了细胞和培养基蛋白质合成的pH依赖性。结果表明，最大蛋白质合成发生在接近生理pH值时。在较低和较高pH值时，蛋白质合成均减少。添加乙醇（30 mM）可抑制蛋白质合成。随着肝细胞悬液pH值降低，乙醇引起的相对抑制作用更加明显。乙醇代谢导致细胞悬液pH值降低。提示乙醇对蛋白质合成的抑制作用可能是由乙醇代谢导致的pH值下降介导的。
