College of Life Sciences, Henan Agricultural University, 450002, Zhengzhou, China.
Mol Biol Rep. 2022 May;49(5):3765-3772. doi: 10.1007/s11033-022-07216-4. Epub 2022 Mar 17.
Interleukin-1 beta (IL-1β) is a crucial cytokine that has been implicated in cancer and metastasis development. However, its possible mechanistic role in cervical cancer remains unclear. This study aimed to investigate the functions of exogenous IL-1β in cervical cancer cell proliferation and migration.
HeLa cell proliferation and migration were measured using MTT and Transwell assays. A lentivirus-mediated packaging system was used to construct an IL-1β overexpressing cell line. MEK/ERK signal transduction was inhibited by pretreatment with the MEK inhibitor PD98059. qRT-PCR and Western blotting were used to test the expression of relevant genes.
Exogenous IL-1β promoted the proliferation and migration of HeLa cells. In addition, overexpression of IL-1β in HeLa cells promoted cell proliferation. Mechanistically, exogenous IL-1β increased the phosphorylated MEK and ERK levels in HeLa cells and the expression of JUN, RELB, and NF-κB2. Alternatively, blockade of MEK inhibited the promoting proliferation effects of IL-1β and the expression of JUN, RELB, and NF-κB2.
Our data suggest that exogenous IL-1β regulates HeLa cell functions by regulating the MEK/ERK signaling pathway and by targeting JUN, RELB, and NF-κB2. Our study uncovered a potential association across IL-1β, cervical tumor development, and cancer progression.
白细胞介素-1β（IL-1β）是一种关键的细胞因子，与癌症和转移的发展有关。然而，其在宫颈癌中可能的机制作用尚不清楚。本研究旨在探讨外源性 IL-1β在宫颈癌细胞增殖和迁移中的作用。
采用 MTT 和 Transwell 检测 HeLa 细胞增殖和迁移。使用慢病毒介导的包装系统构建 IL-1β过表达细胞系。用 MEK 抑制剂 PD98059 预处理抑制 MEK/ERK 信号转导。采用 qRT-PCR 和 Western blot 检测相关基因的表达。
外源性 IL-1β促进了 HeLa 细胞的增殖和迁移。此外，IL-1β在 HeLa 细胞中的过表达促进了细胞增殖。机制上，外源性 IL-1β增加了 HeLa 细胞中磷酸化 MEK 和 ERK 的水平以及 JUN、RELB 和 NF-κB2 的表达。相反，MEK 阻断抑制了 IL-1β的促增殖作用以及 JUN、RELB 和 NF-κB2 的表达。
我们的数据表明，外源性 IL-1β通过调节 MEK/ERK 信号通路以及靶向 JUN、RELB 和 NF-κB2 来调节 HeLa 细胞的功能。我们的研究揭示了 IL-1β、宫颈肿瘤发展和癌症进展之间的潜在关联。
