Tao Limin, Liu Sisun, Xiong Juying, Yang Huimin, Wu Yanfang, Xu Anli, Gong Yanfeng
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanchang University No. 17, Yong Wai Zheng Road, Nangchang 330006, China.
Department of Geriatrics, The First Affiliated Hospital of Nanchang University No. 17, Yong Wai Zheng Road, Nangchang 330006, China.
Int J Clin Exp Pathol. 2021 Apr 15;14(4):426-433. eCollection 2021.
Cervical cancer is a malignancy with high morbidity and mortality among women. Interleukin (IL)-1β, chemokine (C-C motif) ligand 2 (CCL-2), and activation of NF-κB have been proven to be closely related to the progression of various tumors. However, their role in cervical cancer remains unclear. Cell proliferation, migration, and invasion were detected using MTT, wound healing, and transwell assays. Western blotting and qRT-PCR were used to measure expression of target genes. IL-1β greatly promoted the release of CCL-2 from HeLa cells. Activation of NF-κB and phosphorylated NF-κB (p65) nuclear translocation were accelerated by IL-1β. TPCA-1, a blocker of NF-κB, significantly inhibited the release of CCL-2 from HeLa cells. TPCA-1 markedly reversed the promotional effect of IL-1β on viability of HeLa cells. IL-1β increased the cell migration, proliferation, and invasion of HeLa cells through targeting the NF-κB/CCL-2 pathway. IL-1β/NF-κB/CCL-2 might be a promising treatment target for cervical cancer treatment and prevention.
宫颈癌是一种在女性中发病率和死亡率都很高的恶性肿瘤。白细胞介素（IL）-1β、趋化因子（C-C基序）配体2（CCL-2）以及核因子κB（NF-κB）的激活已被证明与各种肿瘤的进展密切相关。然而，它们在宫颈癌中的作用仍不清楚。使用MTT法、伤口愈合实验和Transwell实验检测细胞增殖、迁移和侵袭情况。采用蛋白质免疫印迹法（Western blotting）和实时定量聚合酶链反应（qRT-PCR）检测靶基因的表达。IL-1β极大地促进了HeLa细胞中CCL-2的释放。IL-1β加速了NF-κB的激活以及磷酸化NF-κB（p65）的核转位。NF-κB阻滞剂TPCA-1显著抑制了HeLa细胞中CCL-2的释放。TPCA-1明显逆转了IL-1β对HeLa细胞活力的促进作用。IL-1β通过靶向NF-κB/CCL-2信号通路增加了HeLa细胞的迁移、增殖和侵袭能力。IL-1β/NF-κB/CCL-2可能是宫颈癌治疗和预防的一个有前景的治疗靶点。
