Siar C H
Arch Oral Biol. 1986;31(6):387-90. doi: 10.1016/0003-9969(86)90162-7.
The coronal dentine of 3 teeth from dentinogenesis imperfecta (DI) type I, 9 teeth from DI type II and 4 controls were examined by a quantitative histological technique. In each case, two representative demineralized sections, one stained in H + E and the other in Schmorl's picrothionin were used. The relative amount of dentinal tubule, atubular dentine and canals/clefts were assessed using the point-counting method. Three basic patterns of distribution of tubules were observed. Pattern 1 formed the largest group and showed a gradual decrease in tubule count from enamel-dentine junction (EDJ) pulpwards, pattern 2 was characterized by a drop in tubule count approximately midway between EDJ and pulpal border, and pattern 3 exhibited a gradual increase in tubule score as the pulp was approached. At the 5 per cent level, both patterns 1 and 2 were found to be statistically significant. Pattern 3 was statistically insignificant for the test specimens and highly significant for the controls. The variation in the distribution of the tubules in coronal dentine in this study indirectly supports the concept of abnormal dentinogenesis in DI attributable to a diminution or lack of normal functional odontoblasts.
采用定量组织学技术检查了3颗I型牙本质发育不全（DI）牙齿、9颗II型DI牙齿的冠状牙本质以及4颗对照牙齿。在每种情况下，均使用了两个代表性的脱矿切片，一个用苏木精和伊红（H + E）染色，另一个用施莫尔氏苦味硫堇染色。使用点计数法评估牙本质小管、无小管牙本质以及管腔/裂隙的相对含量。观察到小管分布的三种基本模式。模式1构成最大的组，显示从小管牙本质交界处（EDJ）向牙髓方向小管数量逐渐减少；模式2的特征是在EDJ与牙髓边界之间的大约中间位置小管数量下降；模式3表现为随着接近牙髓，小管评分逐渐增加。在5%的水平上，发现模式1和模式2均具有统计学意义。模式3对测试标本无统计学意义，而对对照标本具有高度统计学意义。本研究中冠状牙本质中小管分布的变化间接支持了DI中牙本质生成异常是由于正常功能的成牙本质细胞减少或缺乏这一概念。
