Chai Wenqiong, Qu Honglei, Ma Qiugang, Zhu Mingxia, Li Mengmeng, Zhan Yandong, Liu Ziwen, Xu Jing, Yao Huanfen, Li Zeyu, Wang Changfa
Liaocheng Research Institute of Donkey High-efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Anim Biotechnol. 2023 Nov;34(5):1786-1795. doi: 10.1080/10495398.2022.2050920. Epub 2022 Mar 18.
The main component of donkey meat is skeletal muscle, and different muscle fibers have been found to be associated with meat quality. However, RNA-seq technology has yet to be used to profile the transcriptomic changes of different muscles of the donkey. In this study, the characterizations of different muscles on the gene expression profiles of Dezhou donkey were obtained, the aim was to identify the important genes in donkey muscles, and aid in improving donkey meat quality via RNA-seq. In the donkey gluteus (DG) and donkey longissimus dorsi (DL) group, GO enrichment indicated that DEGs were mainly involved in the biological regulation and metabolic process, and KEGG analysis shows that a total of 427 DEGs were mapped to 216 KEGG pathways and 23 KEGG pathways were significantly enriched such as the ribosome, glycolysis/gluconeogenesis, glucagon signaling pathway and biosynthesis of amino acids pathways. Meanwhile, 504 DEGs were mapped to 223 KEGG pathways, in which 17 were significantly enriched including cardiac muscle contraction and oxytocin signaling pathway in donkey hamstring muscles (DH) and DL group. In addition, the tenderness in donkey meat might involve muscle fiber type and glucose metabolism, which might profit from the DEGs including , and . The genes found in this study will provide some ideas for further understanding the molecular mechanism of donkey meat quality.
驴肉的主要成分是骨骼肌，并且已发现不同的肌纤维与肉质有关。然而，RNA测序技术尚未用于分析驴不同肌肉的转录组变化。在本研究中，获得了德州驴不同肌肉在基因表达谱上的特征，目的是鉴定驴肌肉中的重要基因，并通过RNA测序帮助改善驴肉品质。在驴臀肌（DG）和驴背最长肌（DL）组中，基因本体（GO）富集表明差异表达基因（DEGs）主要参与生物调节和代谢过程，京都基因与基因组百科全书（KEGG）分析表明，共有427个DEGs被映射到216条KEGG通路，23条KEGG通路显著富集，如核糖体、糖酵解/糖异生、胰高血糖素信号通路和氨基酸生物合成通路。同时，504个DEGs被映射到223条KEGG通路，其中17条显著富集，包括驴腿筋肌肉（DH）和DL组中的心肌收缩和催产素信号通路。此外，驴肉的嫩度可能涉及肌纤维类型和葡萄糖代谢，这可能受益于包括……在内的DEGs。本研究中发现的基因将为进一步了解驴肉品质的分子机制提供一些思路。
