School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
Food Funct. 2022 Apr 20;13(8):4302-4314. doi: 10.1039/d1fo02450g.
The results of our previous study showed that the structure and function of β-glucan in barley were changed after fermentation by L. DY-1. In this study, the antioxidant activities of RBG (regular barley β-glucan, unfermented) and FBG (barley β-glucan, fermented with L. DY-1) were evaluated by adopting an animal model, (). We also carried out an integrated transcriptomic and metabolomic profiling for RBG and FBG to delineate their signature pathways. RBG treatment has better effects on SOD enzyme activity and ROS levels than FBG, while FBG treatment has better effects on the CAT enzyme activity and MDA content than RBG in . Transcription group analysis showed that FBG mainly decreases the expression of the gene to inhibit the calcium signaling pathway, promotes the Wnt signaling pathway by up-regulating the gene and improving the oxidative damage of ; RBG mainly inhibits calcium signal pathways by reducing the expression of ANT-solute carrier family 25 genes, promoting life adjustment pathways by reducing the expression of the gene to improve the oxidative stress of . Joint analysis showed that the difference between FBG and RBG in the regulation of oxidative stress is mainly reflected in the metabolism pathway of arachidonic acid. Under the regulation of FBG, the expression of the gene was decreased, the expression of leukotriene A4, prostaglandin G2, arachidonic acid and phosphatidylcholine was decreased, and the expression of 14,15-DiHETrE was increased. Under the regulation of RBG, the expression of gene was up-regulated, the expression of metabolites such as leukotriene B4 was up-regulated, and the expression of arachidonic acid and phosphatidylcholine was down-regulated.
我们之前的研究结果表明，经过 L. DY-1 发酵后，大麦中β-葡聚糖的结构和功能发生了变化。在这项研究中，我们采用动物模型（）评估了 RBG（普通大麦β-葡聚糖，未发酵）和 FBG（用 L. DY-1 发酵的大麦β-葡聚糖）的抗氧化活性。我们还对 RBG 和 FBG 进行了综合转录组和代谢组学分析，以描绘它们的特征途径。与 FBG 相比，RBG 处理对 SOD 酶活性和 ROS 水平的影响更好，而 FBG 处理对 CAT 酶活性和 MDA 含量的影响更好。转录组分析表明，FBG 主要通过降低基因的表达来抑制钙信号通路，通过上调基因来促进 Wnt 信号通路，改善；RBG 主要通过降低 ANT 溶质载体家族 25 基因的表达来抑制钙信号通路，通过降低基因的表达来促进生命调节途径，以改善氧化应激。联合分析表明，FBG 和 RBG 在调节氧化应激方面的差异主要反映在花生四烯酸的代谢途径中。在 FBG 的调节下，基因的表达降低，白三烯 A4、前列腺素 G2、花生四烯酸和磷脂酰胆碱的表达降低，14,15-DiHETrE 的表达增加。在 RBG 的调节下，基因的表达上调，白三烯 B4 等代谢物的表达上调，花生四烯酸和磷脂酰胆碱的表达下调。
