School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):1021-1031. doi: 10.1016/j.ijbiomac.2021.11.067. Epub 2021 Nov 16.
β-glucan is an important functional active component with relatively high content in barley. It is reported to possess various biological activities, including anti-oxidative stress, but its mechanism of action remains obscure. In the current study, C. elegans was used as an in vivo animal model to explore its anti-oxidative stress mechanism. We found that both RBG (raw barley β-glucan) and FBG (fermented barley β-glucan) could significantly reduce the ROS level in C. elegans under oxidative emergency conditions. In addition, both FBG and RBG had positive effects on SOD and CAT enzyme activity, and FBG treatment obviously reduced the MDA content in nematodes under oxidative stress. Moreover, FBG and RBG pretreatment could extend the median lifespan of C. elegans under oxidative stress. The CB1370 and CF1038 mutants further confirmed that daf-2 and daf-16 were necessary for FBG or RBG to participate in anti-oxidative stress, and the RT-PCR results also evidenced that β-glucans resist oxidative stress in C. elegans partially through the daf-2/daf-16 pathway. In summary, barley β-glucan has high potential to defense oxidative stress as a natural polysaccharide.
β-葡聚糖是大麦中含量较高的一种重要功能活性成分。据报道，它具有多种生物活性，包括抗氧化应激作用，但作用机制尚不清楚。本研究采用秀丽隐杆线虫作为体内动物模型，探讨其抗氧化应激机制。结果发现，无论是 RBG（生大麦β-葡聚糖）还是 FBG（发酵大麦β-葡聚糖），在氧化应激条件下，均能显著降低秀丽隐杆线虫体内的 ROS 水平。此外，FBG 和 RBG 对 SOD 和 CAT 酶活性均有积极影响，FBG 处理可明显降低线虫在氧化应激下的 MDA 含量。而且，FBG 和 RBG 预处理可延长秀丽隐杆线虫在氧化应激下的中位寿命。CB1370 和 CF1038 突变体进一步证实，FBG 或 RBG 参与抗氧化应激需要 daf-2 和 daf-16，RT-PCR 结果也表明β-葡聚糖在秀丽隐杆线虫中部分通过 daf-2/daf-16 途径抵抗氧化应激。综上所述，大麦β-葡聚糖作为一种天然多糖，具有较高的防御氧化应激的潜力。
