Department of Clinical Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Fatima Albohairy, Electron Microscope Research Unit, Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
PLoS One. 2022 Mar 18;17(3):e0265226. doi: 10.1371/journal.pone.0265226. eCollection 2022.
Nickel-titanium (NiTi) instruments used to treat root canal infections are affected by autoclave sterilization in various ways. The aim of this study was to compare the effect of autoclave sterilization on two NiTi rotary instruments that undergo different manufacturing treatments: The electro-polished Race and the heat-treated Race Evo, using scanning electron microscope analysis. In this in-vitro study, Race and Race-Evo instruments were subjected to a number of autoclaving cycles (0, 1, 3, 5, and 10). Scanning electron microscopy images were obtained at 3 mm from the tip of the file at 450x and 1000x magnifications. Surface roughness parameters were measured using ImageJ software. The results showed that autoclave sterilization caused a significant decrease in conventional NiTi Race surface roughness. While in Race Evo, surface roughness increased following the first autoclaving cycle. After 10 autoclaving cycles, surface roughness significantly decreased for both Race and Race Evo files.
镍钛(NiTi)器械常用于治疗根管感染,其受高压蒸汽灭菌的影响各有不同。本研究旨在比较两种不同制造处理方式的镍钛旋转器械在经过高压蒸汽灭菌后的效果,这两种器械分别是经过电化学抛光处理的 Race 以及经过热处理的 Race Evo,采用扫描电子显微镜分析。在这项体外研究中,将 Race 和 Race-Evo 器械分别进行了 0、1、3、5 和 10 次的高压蒸汽灭菌循环。在距器械尖端 3mm 处,使用扫描电子显微镜以 450x 和 1000x 放大倍数获得图像。使用 ImageJ 软件测量表面粗糙度参数。结果表明,高压蒸汽灭菌会显著降低传统镍钛 Race 的表面粗糙度。而在 Race Evo 中,表面粗糙度在第一次高压蒸汽灭菌循环后增加。经过 10 次高压蒸汽灭菌循环后,Race 和 Race Evo 器械的表面粗糙度均显著降低。