Brown L A, Longmore W J
Biochim Biophys Acta. 1986 Sep 12;878(2):258-65. doi: 10.1016/0005-2760(86)90154-2.
To study the effect of diabetes on pulmonary surfactant secretion, type II pneumocytes from adult streptozotocin-induced diabetic rats were placed in short-term culture. As opposed to a linear secretory rate by control type II cells, the secretory rate of type II cells from diabetic animals was biphasic reaching a minimum at 1.5 h. When exogenous surfactant containing radioactive phosphatidylcholine was added to the incubation media for 1.5 h, the cells from diabetic animals incorporated more exogenous phosphatidylcholine into lamellar bodies than control cells. This suggests that in the type II cell from diabetic animals, the rate of reutilization is greater than the rate of secretion until 1.5 h, at which time the rate of secretion becomes greater. The altered secretory pattern was reversed by in vivo insulin treatment 30 min prior to killing but not by the addition of insulin to the incubation media. When challenged by isoproterenol, a beta-adrenergic agonist, the secretory pattern of cells from diabetic animals was biphasic as observed with basal secretion; however, secretion was stimulated 30% as opposed to 100% increase in control cells. These data suggest that basal and stimulated secretion are altered in the cultured type II cell from diabetic animals and restored by in vivo but not in vitro insulin treatment.
为研究糖尿病对肺表面活性物质分泌的影响，将成年链脲佐菌素诱导的糖尿病大鼠的II型肺细胞进行短期培养。与对照II型细胞的线性分泌速率不同，糖尿病动物的II型细胞分泌速率呈双相性，在1.5小时达到最小值。当将含有放射性磷脂酰胆碱的外源性表面活性物质添加到孵育培养基中1.5小时时，糖尿病动物的细胞比对照细胞将更多的外源性磷脂酰胆碱掺入板层小体中。这表明在糖尿病动物的II型细胞中，直到1.5小时再利用速率大于分泌速率，此时分泌速率变得更大。在处死前30分钟进行体内胰岛素治疗可逆转改变的分泌模式，但向孵育培养基中添加胰岛素则不能。当用β-肾上腺素能激动剂异丙肾上腺素刺激时，糖尿病动物细胞的分泌模式与基础分泌时一样呈双相性；然而，分泌受到刺激，增加了30%，而对照细胞增加了100%。这些数据表明，糖尿病动物培养的II型细胞的基础分泌和刺激分泌发生改变，并且通过体内而非体外胰岛素治疗得以恢复。
