Pan Chengyu, Yu Jinxia, Yao Qian, Lin Nan, Lu Zhenping, Zhang Yan, Zhao Shasha, Wang Zixia, Lei Xiaoning, Tian Ying, Gao Yu
Department of Environmental Health, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China (USTC), Anhui, China.
Environ Int. 2022 May;163:107180. doi: 10.1016/j.envint.2022.107180. Epub 2022 Mar 15.
An increasing number of studies have reported neonicotinoid insecticides (NEOs), the emerging alternatives to conventional insecticides, may increase oxidative stress and cause adverse health effects, but limited is known about the prenatal NEOs exposures and their impact on birth outcomes.
We investigated the levels of prenatal exposure to NEOs/metabolites, to assess their associations with birth outcomes, and investigate whether these associations could be mediated by oxidative stress using 8-OHdG as the biomarker.
We studied 296 mother-infant pairs recruited from Laizhou Wan Birth Cohort in 2010 - 2013. Two NEOs (IMI and ACE), three metabolites (6-CN, ND-ACE, and 2CTCA), and 8-OHdG were measured in maternal urine collected before delivery. Birth outcomes including birth weight, birth length, ponderal index (PI), head circumference, and gestational age, were acquired. We examined the associations between NEOs/metabolites and birth outcomes using multivariable linear regression. Mediation analysis was conducted to clarify the role of 8-OHdG on the association of NEOs/metabolites exposure and birth outcomes.
Highest detection rate was observed for ACE (100.0%), followed by IMI (98.3%) and 6-CN (98.0%), suggesting the common exposure of pregnant women. The highest median concentration was observed for 6-CN with creatinine-adjusted median levels of 9.58 μg/g creatinine. A decrease in newborns' head circumference was observed with a 10-fold increase in IMI (β = -1.83; 95% CI = -3.04, -0.62) and ACE (β = -2.27; 95% CI = -3.56, -0.98). An increase in newborns' PI was observed with a 10-fold increase in IMI (β = 0.40; 95% CI = 0.03, 0.75). Maternal 8-OHdG demonstrated 38.5-65.5% mediating effects in the negative association of IMI, ACE, 2-CTCA with head circumference. These associations might differ between boys and girls.
Pregnant women were widely exposed to NEOs/metabolites in China. Results suggested the potential impacts of prenatal exposure to certain neonicotinoid insecticides on head circumference. Urinary 8-OHdG may partly mediate these associations.
越来越多的研究报告称，作为传统杀虫剂新兴替代品的新烟碱类杀虫剂（NEOs）可能会增加氧化应激并导致不良健康影响，但关于产前新烟碱类杀虫剂暴露及其对出生结局的影响知之甚少。
我们调查了产前新烟碱类杀虫剂/代谢物的暴露水平，以评估它们与出生结局的关联，并使用8-羟基脱氧鸟苷（8-OHdG）作为生物标志物来研究这些关联是否可由氧化应激介导。
我们研究了2010年至2013年从莱州湾出生队列招募的296对母婴。在分娩前收集的孕妇尿液中测量了两种新烟碱类杀虫剂（吡虫啉和啶虫脒）、三种代谢物（6-氯烟碱、去甲基啶虫脒和2-氯-5-三氟甲基吡啶羧酸）以及8-OHdG。获取了包括出生体重、出生身长、体重指数（PI）、头围和孕周在内的出生结局。我们使用多变量线性回归研究了新烟碱类杀虫剂/代谢物与出生结局之间的关联。进行中介分析以阐明8-OHdG在新烟碱类杀虫剂/代谢物暴露与出生结局关联中的作用。
啶虫脒的检出率最高（100.0%），其次是吡虫啉（98.3%）和6-氯烟碱（98.0%），表明孕妇普遍暴露。6-氯烟碱的肌酐校正中位数浓度最高，为9.58μg/g肌酐。吡虫啉（β = -1.83；95%CI = -3.04，-0.62）和啶虫脒（β = -2.27；95%CI = -3.56，-0.98）增加10倍时，新生儿头围减小。吡虫啉增加10倍时，新生儿PI增加（β = 0.40；95%CI = 0.03，0.75）。母体8-OHdG在吡虫啉、啶虫脒、2-氯-5-三氟甲基吡啶羧酸与头围的负相关中显示出38.5%至65.5%的中介作用。这些关联在男孩和女孩之间可能有所不同。
在中国，孕妇广泛暴露于新烟碱类杀虫剂/代谢物。结果表明产前暴露于某些新烟碱类杀虫剂对头围有潜在影响。尿8-OHdG可能部分介导这些关联。
