Zhuhai Precision Medical Center, Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Jinan University, Zhuhai 519000, Guangdong, PR China; The Biomedical Translational Research Institute, Jinan University Faculty of Medical Science, Jinan University, Guangzhou 510632, Guangdong, PR China.
School of Stomatology, Jinan University, Guangzhou 510632, Guangdong, PR China.
Ecotoxicol Environ Saf. 2021 Apr 1;212:112001. doi: 10.1016/j.ecoenv.2021.112001. Epub 2021 Feb 3.
Neonicotinoid insecticides (NEOs) are widely used around the world. The distribution of NEOs in paired saliva and periodontal blood samples was not previously documented in China. In this study, the concentrations of six NEOs and three corresponding metabolites were measured in 188 paired saliva and periodontal blood samples collected from South China. NEOs and their metabolites were frequently detected (68-94%) in paired saliva and periodontal blood, with median levels of 0.01-0.99 ng/mL. 1-Methyl-3-(tetrahydro-3-furylmethyl) urea was the most predominant NEO in paired saliva (39%) and periodontal blood (42%). Gender-related differences in NEOs and their metabolite concentrations were found: males showed lower levels than females. We calculated the concentration ratios between saliva and periodontal blood (S/PB ratios), and found that the median S/PB ratios of NEO and their metabolites were higher than 1, indicating that NEOs and their metabolites were easily excreted via saliva. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) was measured in paired saliva and periodontal blood as a marker of oxidative stress. 8-OHdG concentrations in saliva and periodontal blood were significantly and positively correlated (p < 0.05) with the concentrations of most NEOs and their metabolites in saliva and periodontal blood samples. These findings indicated that exposure to NEOs and their metabolites is associated with oxidative stress. This study is the first to report NEOs and their metabolites in paired saliva and periodontal blood samples collected from South China.
新烟碱类杀虫剂（NEOs）在全球范围内广泛使用。在中国，以前没有记录过唾液和牙周血样中 NEOs 的分布情况。在这项研究中，测量了从中国南方采集的 188 对唾液和牙周血样中六种 NEOs 和三种相应代谢物的浓度。NEOs 及其代谢物在唾液和牙周血中经常被检测到（68-94%），中位数水平为 0.01-0.99 ng/mL。1-甲基-3-（四氢-3-呋喃甲基）脲是唾液（39%）和牙周血（42%）中最主要的 NEO。NEOs 和其代谢物的浓度存在性别差异：男性的水平低于女性。我们计算了唾液和牙周血之间的浓度比（S/PB 比值），发现 NEO 和其代谢物的中位数 S/PB 比值高于 1，表明 NEOs 和其代谢物很容易通过唾液排泄。8-羟基-2'-脱氧鸟苷（8-OHdG）作为氧化应激的标志物在唾液和牙周血中进行了测量。唾液和牙周血中的 8-OHdG 浓度与唾液和牙周血中大多数 NEOs 和其代谢物的浓度呈显著正相关（p < 0.05）。这些发现表明，接触 NEOs 和其代谢物与氧化应激有关。本研究首次报道了从中国南方采集的唾液和牙周血样中 NEOs 及其代谢物的分布情况。
