Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, South Korea.
Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju-gun 55365, South Korea.
Poult Sci. 2022 May;101(5):101733. doi: 10.1016/j.psj.2022.101733. Epub 2022 Jan 21.
A surrogate eggshell incubation system is a well-defined method to apply to avian genetic modification. In this study, we tried to investigate whether the egg weight differences between donor and surrogate eggs have an effect on donor viability. The groups were divided by egg weight differences between the donor and surrogate eggs into 4 in each system. The viability at d 4 was evaluated at the end of System II, the embryos alive were transferred into the second surrogate eggshells, and the viability at d 5, 6 was evaluated at early phase of System III. Then, the viability of System III was evaluated at different incubation period: d 6-12, d 13-18, d 19-21, and hatching rate was evaluated at d 22. Although the effect of egg weight differences between the donor and surrogate eggs was not observed, a specific group in System III showed higher survival and hatching rate than other group (P > 0.05).
代孕蛋壳孵化系统是一种将禽类遗传修饰应用于医学领域的成熟方法。在本研究中，我们试图探讨供体蛋和代孕蛋之间的蛋重差异是否会对供体的存活能力产生影响。根据供体蛋和代孕蛋之间的蛋重差异，将各组分为 4 组，每组在两个系统中进行实验。在系统 II 结束时评估第 4 天的存活能力，将存活的胚胎转移到第二个代孕蛋壳中，并在系统 III 的早期阶段评估第 5 天和第 6 天的存活能力。然后，评估系统 III 的不同孵化期（第 6-12 天、第 13-18 天、第 19-21 天）的存活能力，并在第 22 天评估孵化率。尽管未观察到供体蛋和代孕蛋之间的蛋重差异的影响，但系统 III 中的一个特定组显示出比其他组更高的存活率和孵化率（P>0.05）。
