Mokkala Kati, Gustafsson Johanna, Vahlberg Tero, Vreugdenhil Anita C E, Ding Lingling, Shiri-Sverdlov Ronit, Plat Jogchum, Laitinen Kirsi
Institute of Biomedicine, Integrative Physiology and Pharmacology, University of Turku, Turku, Finland.
Institute of Clinical Medicine, Biostatistics, University of Turku, Turku, Finland.
Nutr Metab Cardiovasc Dis. 2022 May;32(5):1292-1300. doi: 10.1016/j.numecd.2022.02.011. Epub 2022 Feb 23.
Elevated circulating levels of CathepsinD (CatD) have been linked to metabolic deviations including liver inflammation. We investigated 1) whether supplementation with probiotics and/or fish oil affects CatD and 2) whether the CatD concentration would associate with gestational diabetes (GDM), low-grade inflammation, lipid metabolism, body fat % and dietary composition.
Overweight/obese pregnant women (n = 438) were randomized into fish oil + placebo, probiotics + placebo, fish oil + probiotics or placebo + placebo groups. Fish oil contained 1.9 g docosahexaenoic acid and 0.22 g eicosapentaenoic acid and probiotics were Lacticaseibacillusrhamnosus HN001 (formerly Lactobacillusrhamnosus HN001) and Bifidobacteriumanimalis ssp. lactis 420, 10 colony-forming units each). Serum CatD levels were analysed by ELISA, GlycA and lipid metabolites by NMR, high sensitive C-reactive protein (hsCRP) by immunoassay, and intakes of energy yielding nutrients and n-3 and n-6 fatty acids from food diaries at both early and late pregnancy. GDM was diagnosed by OGTT. CatD concentrations did not differ between the intervention groups or by GDM status. Multivariable linear models revealed that body fat % and GlycA affected CatD differently in healthy women and those with GDM.
The serum CatD concentration of pregnant women was not modified by this dietary intervention. Serum CatD was influenced by two parameters, body fat and low grade inflammation, which were dependent on the woman's GDM status. CLINICAL TRIAL REG. NO: NCT01922791, clinicaltrials.gov (secondary analysis).
组织蛋白酶D（CatD）循环水平升高与包括肝脏炎症在内的代谢异常有关。我们研究了1）补充益生菌和/或鱼油是否会影响CatD，以及2）CatD浓度是否与妊娠期糖尿病（GDM）、低度炎症、脂质代谢、体脂百分比和饮食组成相关。
超重/肥胖孕妇（n = 438）被随机分为鱼油 + 安慰剂、益生菌 + 安慰剂、鱼油 + 益生菌或安慰剂 + 安慰剂组。鱼油含有1.9克二十二碳六烯酸和0.22克二十碳五烯酸，益生菌为鼠李糖乳杆菌HN001（原鼠李糖乳杆菌HN001）和动物双歧杆菌乳亚种420（各10个菌落形成单位）。通过酶联免疫吸附测定法分析血清CatD水平，通过核磁共振分析糖基化终末产物（GlycA）和脂质代谢物，通过免疫测定法分析高敏C反应蛋白（hsCRP），并通过早孕期和晚孕期的食物日记记录能量产生营养素以及n-3和n-6脂肪酸的摄入量。通过口服葡萄糖耐量试验（OGTT）诊断GDM。各干预组之间或不同GDM状态下的CatD浓度无差异。多变量线性模型显示，健康女性和患有GDM的女性的体脂百分比和GlycA对CatD的影响不同。
这种饮食干预未改变孕妇的血清CatD浓度。血清CatD受两个参数影响，即体脂和低度炎症，这两个参数取决于女性的GDM状态。临床试验注册号：NCT01922791，clinicaltrials.gov（二次分析）。
