Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, PR China.
Chemosphere. 2022 Jul;299:134333. doi: 10.1016/j.chemosphere.2022.134333. Epub 2022 Mar 15.
Soils containing both veterinary antibiotics (VAs) and heavy metals necessitate effective remediation approaches, and microbial and molecular levels of the results should be further examined. Here, a novel material combining waste fungus chaff-based biochar (WFCB) and Herbaspirillum huttiense (HHS1) was established to immobilize copper (Cu) and zinc (Zn) and degrade oxytetracycline (OTC) and enrofloxacin (ENR). Results showed that the combined material exhibited high immobilization of Cu (85.5%) and Zn (64.4%) and great removals of OTC (41.9%) and ENR (40.7%). Resistance genes including tet(PB), tetH, tetR, tetS, tetT, tetM, aacA/aphD, aacC, aadA9, and czcA were reduced. Abundances of potential hosts of antibiotic resistance genes (ARGs) including phylum Proteobacteria and genera Brevundimonas and Rhodanobacter were altered. Total phosphorus and pH were the factors driving the VA degrading microorganisms and potential hosts of ARGs. The combination of WFCB and HHS1 can serve as an important bioresource for immobilizing heavy metals and removing VAs in the contaminated soil.
土壤中同时含有兽用抗生素（VAs）和重金属，需要采取有效的修复方法，并且应该进一步检查微生物和分子水平的结果。在这里，建立了一种将废菌糠基生物炭（WFCB）和希瓦氏菌（HHS1）结合的新型材料，以固定铜（Cu）和锌（Zn）并降解土霉素（OTC）和恩诺沙星（ENR）。结果表明，该组合材料对 Cu（85.5%）和 Zn（64.4%）具有高的固定化作用，对 OTC（41.9%）和 ENR（40.7%）具有很好的去除作用。包括 tet(PB)、tetH、tetR、tetS、tetT、tetM、aacA/aphD、aacC、aadA9 和 czcA 在内的抗性基因减少。抗生素抗性基因（ARGs）的潜在宿主包括门变形菌门和 Brevundimonas 和 Rhodanobacter 属的丰度发生了变化。总磷和 pH 值是驱动 VA 降解微生物和 ARGs 潜在宿主的因素。WFCB 和 HHS1 的组合可以作为一种重要的生物资源，用于固定污染土壤中的重金属和去除 VAs。
