Department of Physics, Dankook University, Cheonan, 31116, Republic of Korea.
Research Institute of Natural Science and Department of Physics Education, Gyeongsang National University, Jinju, 52828, Republic of Korea.
Photodiagnosis Photodyn Ther. 2022 Jun;38:102812. doi: 10.1016/j.pdpdt.2022.102812. Epub 2022 Mar 15.
Photodynamic therapy utilizes light energy with a photosensitizer (a light-sensitive drug) to kill cancer cells through creation of singlet oxygen via light activation. When a photosensitizer is injected into the bloodstream and exposed to a specific wavelength of light, it generates oxygen to destroy or damage nearby cancer cells, while minimizing side effects on normal cells. Although photodynamic therapy is effective for treating cancer, various parameters, such as the optimum light intensity and photosensitizer dose, are currently poorly understood due to the complexity of conventional experimental schemes.
To effectively perform a simultaneous single parallel test for several different light irradiation conditions on each cell, a microfluidic device was developed to generate eight different intensities from a single light-emitting diode source, through eight different color dye concentrations functioning as light intensity filters. To show that this novel high-throughput microfluidic system can analyze the effects of various light intensities during photodynamic therapy, the optimum light intensities and photosensitizer doses were determined for two different cancer cell lines.
Optimum light intensities and photosensitizer were determined for all cell lines. The photodynamic therapy effects in response to different irradiated light intensities were characterized by analyzing cell viability after photosensitizer treatment CONCLUSIONS: : The developed platform is capable of being used as a photodynamic therapy screening tool. The proposed platform provides a simple and robust way to optimize the combined parameters of light intensity and dosage for diverse types of cancer cells.
光动力疗法利用光能和光敏剂（一种光敏感药物）通过光激活产生单线态氧来杀死癌细胞。当光敏剂注入血液并暴露于特定波长的光时，它会产生氧气来破坏或损伤附近的癌细胞，同时最大限度地减少对正常细胞的副作用。尽管光动力疗法对治疗癌症有效，但由于传统实验方案的复杂性，目前对各种参数（如最佳光强度和光敏剂剂量）了解甚少。
为了有效地对每个细胞的几种不同光照射条件进行同时单一平行测试，开发了一种微流控装置，通过八种不同的颜色染料浓度作为光强度滤光片，从单个发光二极管源产生八种不同的强度。为了表明这种新颖的高通量微流控系统可以分析光动力疗法过程中各种光强度的影响，确定了两种不同癌细胞系的最佳光强度和光敏剂剂量。
确定了所有细胞系的最佳光强度和光敏剂。通过分析光敏剂处理后细胞活力来表征不同辐照光强度下的光动力治疗效果。
所开发的平台可用作光动力疗法筛选工具。该平台提供了一种简单而强大的方法，可以优化不同类型癌细胞的光强度和剂量的组合参数。
