Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, China.
Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Luminescence. 2022 Jun;37(6):868-875. doi: 10.1002/bio.4230. Epub 2022 Mar 28.
Sensitive detection of doxorubicin (DOX) is critical for clinical theranostics. A novel ratiometric fluorescence strategy based on the inner filter effect (IFE) has been established for the sensitive detection of DOX by designing a ratiometric fluorescence probe. In the presence of DOX, the fluorescence intensity of copper nanoclusters (CuNCs) at 485 nm decreases, and the fluorescence intensity of carbon dots at 560 nm increases. Therefore, DOX can be quantitatively detected by measuring the ratio of the fluorescence intensities at 560 and 485 nm (F /F ). The F /F ratio exhibits a linear correlation with the DOX concentration in the range from 1.0 × 10 M to 1.0 × 10 M with the detection limit of 3.7 nM. Furthermore, this method was also successfully applied to the analysis of DOX in human plasma samples, affording an effective platform for drug safety management.
阿霉素（DOX）的灵敏检测对于临床治疗至关重要。通过设计比率型荧光探针，建立了一种基于内滤效应（IFE）的新型比率型荧光策略，用于灵敏检测 DOX。在 DOX 存在的情况下，铜纳米团簇（CuNCs）在 485nm 处的荧光强度降低，而碳点在 560nm 处的荧光强度增加。因此，可以通过测量 560nm 和 485nm 处的荧光强度比（F/F）来定量检测 DOX。F/F 比与 DOX 浓度在 1.0×10-7 M 至 1.0×10-6 M 的范围内呈线性相关，检测限为 3.7nM。此外，该方法还成功应用于人血浆样品中 DOX 的分析，为药物安全管理提供了有效的平台。
