Department of Chemistry, Sardar Vallabhbhai National Institute of Technology (SVNIT), Surat, 395007, Gujarat, India.
Department of Chemistry, Veer Surendra Sai University of Technology, Burla, Sambalpur, Odisha, India.
Mikrochim Acta. 2024 Nov 29;191(12):774. doi: 10.1007/s00604-024-06856-6.
Polyethyleneimine (PEI) based fluorescent polymeric nanorods with high quantum yield (87.21%) were synthesized by crosslinking and self-assembly of PEI by vitamin B cofactor pyridoxal 5'-phosphate (PLP). The fluorescent PEIPLP polymeric nanorods were employed for the ratiometric detection of doxorubicin (DOX). With the addition of DOX to the PEIPLP nanorods, the fluorescent intensity was decreased at 510 nm and concomitantly enhanced at 590 nm due to the fluorescence resonance energy transfer (FRET) process. The selective and specific ratiometric fluorescence response from the PEIPLP nanorods can be employed to detect DOX down to 10 nM. The practical utility of the PEIPLP nanorods was examined by detecting DOX in human serum.
通过维生素 B 辅因子吡哆醛 5′-磷酸（PLP）交联和自组装，合成了具有高量子产率（87.21%）的聚乙烯亚胺（PEI）基荧光聚合物纳米棒。荧光 PEIPLP 聚合物纳米棒用于阿霉素（DOX）的比率检测。随着 DOX 加入到 PEIPLP 纳米棒中，由于荧光共振能量转移（FRET）过程，510nm 处的荧光强度降低，同时 590nm 处的荧光强度增强。PEIPLP 纳米棒的选择性和特异性比率荧光响应可用于检测低至 10 nM 的 DOX。通过检测人血清中的 DOX，检验了 PEIPLP 纳米棒的实际应用。
