Mescheryakova Svetlana A, Koganova Daria G, Tsyupka Daria V, Kornilov Danila A, Pidenko Pavel S, Drozd Daniil D, Goryacheva Olga A, Goryacheva Irina Yu
Saratov State University Named After N.G. Chernyshevsky, Astrakhanskaya 83, 410012, Saratov, Russia.
Mikrochim Acta. 2025 Jun 12;192(7):416. doi: 10.1007/s00604-025-07283-x.
The feasibility of doxorubicin (Dox) detection in plasma and blood using a luminescence turn-off nanosensor based on alloyed CdZnSeS/ZnS quantum dots (QDs) is demonstrated. The QD luminescence quenching in the presence of Dox was carefully examined. Optimal assay conditions were selected, including plasma dilution and QD concentration. The calculated limit of detection value was 0.02 µg/mL. Time-resolved QD luminescence quenching and absorption measurements allowed us to determine that the quenching process occurs via a combined mechanism. At low Dox concentrations, the dynamic quenching is predominant, while at Dox concentrations above 2.5 μM, the contribution of static quenching increases drastically. Dox detection in whole human blood was performed and compared with other methods for the drug detection in blood. The results of the method are comparable with those of the high-performance liquid chromatography with ultraviolet-visible detection.
本文展示了基于合金化CdZnSeS/ZnS量子点（QD）的发光猝灭纳米传感器用于检测血浆和血液中阿霉素（Dox）的可行性。仔细研究了阿霉素存在下量子点发光的猝灭情况。选择了最佳检测条件，包括血浆稀释度和量子点浓度。计算得出的检测限为0.02μg/mL。通过时间分辨量子点发光猝灭和吸收测量，我们确定猝灭过程是通过一种联合机制发生的。在低阿霉素浓度下，动态猝灭占主导，而在阿霉素浓度高于2.5μM时，静态猝灭的贡献急剧增加。对全血中的阿霉素进行了检测，并与其他血液中药物检测方法进行了比较。该方法的结果与高效液相色谱-紫外可见检测法的结果相当。
