Hirokawa K, Kubo S, Utsuyama M, Kurashima C, Sado T
Cell Immunol. 1986 Jul;100(2):443-51. doi: 10.1016/0008-8749(86)90043-2.
Bone marrow chimeras were produced between various combinations of young and old mice using either C57BL/6 mice only or a combination of C57BL/6 and B10.Thy-1.1 mice. The wet weight of the thymus and the number of thymocytes and splenic T cells of donor origin were assessed at appropriate intervals after the bone marrow transplantation. It was revealed that the old bone marrow was inferior to young in terms of the capacity to repopulate the thymus and splenic T cells. Moreover, some age-related qualitative changes appeared to occur in the thymocyte progenitors, as the composition of Lyt phenotype of donor-type T cells in the spleen was different between chimeras produced with young bone marrow and those with old.
利用仅C57BL/6小鼠或C57BL/6与B10.Thy-1.1小鼠的组合，在年轻和年老小鼠的各种组合之间产生骨髓嵌合体。在骨髓移植后的适当时间间隔评估胸腺的湿重以及供体来源的胸腺细胞和脾T细胞的数量。结果显示，就重新填充胸腺和脾T细胞的能力而言，年老骨髓不如年轻骨髓。此外，胸腺细胞祖细胞似乎发生了一些与年龄相关的质性变化，因为用年轻骨髓产生的嵌合体与用年老骨髓产生的嵌合体相比，脾脏中供体型T细胞的Lyt表型组成不同。
