Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Biomed Chromatogr. 2022 Jul;36(7):e5371. doi: 10.1002/bmc.5371. Epub 2022 Mar 29.
A dispersive solid-phase extraction method based on a new sorbent has been performed on plasma and wastewater samples to determine metoprolol by high-performance liquid chromatography-tandem mass spectrometry. In this study, the analyte was adsorbed from the samples onto microcrystalline cellulose as a green and efficient sorbent and then eluted for use in the determination step. In the mass spectrometer, the analyte was detected in the positive mode and selectivity of the analysis was increased by sequential mass analysis through multiple reaction monitoring. All of the effective parameters in the extraction of metoprolol from plasma and wastewater were optimized. Under optimal conditions the method was linear in the ranges of 1-1,000 and 0.1-1,000 ng/ml in plasma and wastewater samples, respectively. The detection limits of the method were 0.30 and 0.03 ng/ml in plasma and wastewater samples, respectively. The data showed that the method provides low detection limit, wide linear range, good precision and high extraction recovery. Finally several plasma and wastewater samples were successfully analyzed using the method. The use of a small amount of a green and inexpensive sorbent and a low volume of plasma without the need for further pretreatment steps are the main advantages of the method.
一种基于新型吸附剂的分散固相萃取方法已应用于血浆和废水样品中，通过高效液相色谱-串联质谱法测定美托洛尔。在这项研究中，分析物从样品中吸附到微晶纤维素上，作为一种绿色、高效的吸附剂，然后洗脱用于测定步骤。在质谱仪中，分析物以正模式检测，通过多反应监测进行连续质量分析，提高了分析的选择性。优化了从血浆和废水中提取美托洛尔的所有有效参数。在最佳条件下，该方法在血浆和废水样品中的线性范围分别为 1-1000 和 0.1-1000ng/ml。该方法在血浆和废水样品中的检测限分别为 0.30 和 0.03ng/ml。数据表明，该方法具有低检测限、宽线性范围、良好的精密度和高提取回收率。最后，使用该方法成功分析了几个血浆和废水样品。该方法的主要优点是使用少量绿色、廉价的吸附剂和少量未经进一步预处理的血浆。
