Biophysical and Protein Chemistry Laboratory, Department of Chemistry, NIT Rourkela, Rourkela, India.
Department of Science, Roma Tre University, Rome, Italy.
Luminescence. 2022 Jun;37(6):876-882. doi: 10.1002/bio.4231. Epub 2022 Apr 13.
A comparative study of interaction between chicken egg white lysozyme (Lyz) with two hexavalent chromate ions; chromate and dichromate; which are prevalently known for their toxicity, was investigated using different spectroscopic techniques along with a molecular docking study. Both steady-state and time-resolved studies revealed that the addition of chromate/dichromate is responsible for strong quenching of intrinsic fluorescence in Lyz and the quenching is caused by both static and dynamic quenching mechanisms. Different binding and thermodynamic parameters were also calculated at different temperatures from the intrinsic fluorescence of Lyz. The conformational change in Lyz and thermodynamic parameters obtained during the course of interaction with chromate/dichromate were well-supported by the molecular docking results.
采用不同光谱技术结合分子对接研究，研究了鸡蛋白溶菌酶（Lyz）与两种六价铬离子（铬酸盐和重铬酸盐）之间的相互作用。这两种离子普遍具有毒性。稳态和时间分辨研究表明，铬酸盐/重铬酸盐的加入导致 Lyz 中固有荧光的强烈猝灭，猝灭是由静态和动态猝灭机制引起的。还从 Lyz 的固有荧光中计算了不同温度下的不同结合和热力学参数。Lyz 与铬酸盐/重铬酸盐相互作用过程中构象变化和热力学参数得到了分子对接结果的很好支持。
