School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
Res Vet Sci. 2022 Sep;146:24-27. doi: 10.1016/j.rvsc.2022.03.002. Epub 2022 Mar 8.
Circular RNAs (circRNAs) are widely involved in inflammatory responses, but their specific regulatory roles in cow mastitis remain controversial. In this study, RNA-seq was used to generate a circRNA expression profile, which identified 71 differentially expressed circRNAs (DEcircRNAs) in lipopolysaccharide (LPS)-stimulated MAC-T bovine mammary epithelial cells (bMECs) at different stages of inflammation. Functional analyses revealed that these DEcircRNAs may be involved in cellular proliferation, apoptosis, migration, and the inflammatory responses through regulation of numerous related signaling pathways. In addition, these data suggest that 2 novel circRNAs, named novel_circ_0004830 and novel_circ_0003097, may act as the key competing endogenous RNAs (ceRNAs) in the regulation of bovine mastitis through binding to inflammation-related microRNAs (miRNAs). These results provide a new angle for the study of the molecular regulatory mechanisms in dairy cow mastitis.
环状 RNA（circRNAs）广泛参与炎症反应，但它们在奶牛乳腺炎中的具体调节作用仍存在争议。在这项研究中，我们使用 RNA-seq 生成了一个 circRNA 表达谱，该表达谱在脂多糖（LPS）刺激的不同炎症阶段的 MAC-T 奶牛乳腺上皮细胞（bMEC）中鉴定出 71 个差异表达的 circRNAs（DEcircRNAs）。功能分析表明，这些 DEcircRNAs 可能通过调节许多相关信号通路，参与细胞增殖、凋亡、迁移和炎症反应。此外，这些数据表明，2 个新型 circRNAs，命名为 novel_circ_0004830 和 novel_circ_0003097，可能通过与炎症相关 microRNAs（miRNAs）结合，作为调控奶牛乳腺炎的关键竞争性内源 RNA（ceRNA）。这些结果为研究奶牛乳腺炎的分子调控机制提供了一个新的视角。
