State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China.
J Environ Sci (China). 2022 Aug;118:130-139. doi: 10.1016/j.jes.2021.08.038. Epub 2022 Jan 11.
Ecological restoration is one of the hot technologies for the reconstruction of eutrophic lake ecosystems in which the restoration and propagation of submerged plants is the key and difficult step. In this paper, the effect of vermiculite on the growth process of Vallisneria spiralis and sediment microenvironment were investigated, aiming to provide a theoretical basis for the application of vermiculite in aquatic ecological restoration. Results of growth indexes demonstrated that 5% and 10% vermiculite treatment groups statistically promote the growth of Vallisneria spiralis compared to the control. Meanwhile, the results of ecophysiological indexes showed that photosynthetic pigment, soluble sugar content, superoxide dismutase (SOD), and catalase (CAT) activity of 5% and 10% group were increased compared with the control while the malondialdehyde (MDA) content exhibited the opposite result (p < 0.05), which illustrated that vermiculite can improve the resistance of plants and delay the aging process of Vallisneria spiralis. In addition, result of PCA (Principal Component Analysis) demonstrated 5% and 10% group has improved the sediment physical conditions and create more ecological niche for microorganisms directly, and then promoted the growth of plants. The dissolution results showed that vermiculite can dissolve the constant and trace elements needed for plant growth. Furthermore, the addition of vermiculite increased the diversity of microorganisms in the sediments, and promoted the increase of plant growth-promoting bacteria and phosphorus-degrading bacteria. This study could provide a technique reference for the further application of vermiculite in the field of ecological restoration.
生态修复是富营养化湖泊生态系统重建的热门技术之一，其中沉水植物的恢复和繁殖是关键和难点步骤。本研究旨在探讨蛭石对苦草生长过程和底泥微环境的影响，为蛭石在水生态修复中的应用提供理论依据。生长指标结果表明，与对照组相比，5%和 10%蛭石处理组的苦草生长具有统计学意义的促进作用。同时，生理生态指标结果表明，5%和 10%组的光合色素、可溶性糖含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性均高于对照组，而丙二醛（MDA）含量则相反（p<0.05），这表明蛭石可以提高植物的抗性，延缓苦草的衰老过程。此外，PCA（主成分分析）结果表明，5%和 10%组直接改善了底泥的物理条件，为微生物创造了更多的生态位，从而促进了植物的生长。溶出结果表明，蛭石可以溶解植物生长所需的常量和微量元素。此外，添加蛭石增加了沉积物中微生物的多样性，并促进了植物促生菌和磷降解菌的增加。本研究可为蛭石在生态修复领域的进一步应用提供技术参考。
