Luo Shouyang, Tian Chunjie, Zhang Hengfei, Yao Zongmu, Guan Zhihui, Li Yingxin, Zhang Jianfeng, Song Yanyu
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Key Laboratory of Straw Comprehensive Utilization and Black Soil, Conservation College of Life Science, The Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Microorganisms. 2023 Sep 25;11(10):2384. doi: 10.3390/microorganisms11102384.
Fusarium root rot (FRR) seriously affects the growth and productivity of . Therefore, protecting from FRR has become an important task, especially for increasing production. The purpose of this study was to screen FRR control strains from the rhizosphere soil. Eighty-four bacterial strains and seven fungal strains were isolated, and five strains were identified with high inhibitory effects against (FO): (MH), (CJ5, CJ7, and CJ8), and (CJ9). All five strains had high antagonistic effects in vitro. Results showed that MH and CJ5, as biological control agents, had high control potential, with antagonistic rates of 86.01% and 82.78%, respectively. In the pot experiment, the growth levels of roots and stems of seedlings treated with MH+CJ were significantly higher than those of control plants. The total nitrogen, total phosphorus, total potassium, indoleacetic acid, and chlorophyll contents in leaves were also significantly increased. In the biocontrol test, the combined MH + CJ application significantly decreased the malondialdehyde content in roots and significantly increased the polyphenol oxidase, phenylalanine ammonolyase, and peroxidase ability, indicating a high biocontrol effect. In addition, the application of spp. and increased the abundance and diversity of the soil fungal population, improved the soil microbial community structure, and significantly increased the abundance of beneficial strains, such as and . The abundance of , , and other pathogenic strains was significantly reduced, and the biocontrol potential of root rot was increased. Thus, spp. and complex bacteria can be considered potential future biocontrol agents for FRR.
镰刀菌根腐病（FRR）严重影响[作物名称未给出]的生长和生产力。因此，保护[作物名称未给出]免受FRR侵害已成为一项重要任务，特别是对于提高[作物名称未给出]产量而言。本研究的目的是从[作物名称未给出]根际土壤中筛选出控制FRR的菌株。分离出了84株细菌菌株和7株真菌菌株，并鉴定出5株对[病原菌名称未给出]（FO）具有高抑制作用的菌株：[菌株名称未给出]（MH）、[菌株名称未给出]（CJ5、CJ7和CJ8）以及[菌株名称未给出]（CJ9）。所有这5株菌株在体外均具有高拮抗作用。结果表明，作为生物防治剂的MH和CJ5具有很高的防治潜力，拮抗率分别为86.01%和82.78%。在盆栽试验中，用MH + CJ处理的[作物名称未给出]幼苗的根和茎的生长水平显著高于对照植株。[作物名称未给出]叶片中的全氮、全磷、全钾、吲哚乙酸和叶绿素含量也显著增加。在生物防治试验中，联合施用MH + CJ显著降低了[作物名称未给出]根中的丙二醛含量，并显著提高了多酚氧化酶、苯丙氨酸解氨酶和过氧化物酶的活性，表明具有很高的生物防治效果。此外，[菌株名称未给出]属和[菌株名称未给出]属细菌的施用增加了土壤真菌种群的丰度和多样性，改善了土壤微生物群落结构，并显著增加了有益菌株如[有益菌株名称未给出]和[有益菌株名称未给出]的丰度。[病原菌名称未给出]、[病原菌名称未给出]等病原菌的丰度显著降低，[作物名称未给出]根腐病的生物防治潜力得到提高。因此，[菌株名称未给出]属和[菌株名称未给出]属复合细菌可被视为未来防治FRR的潜在生物防治剂。
