Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China; Department of Otolaryngology-Head Neck Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Department of Otolaryngology-Head Neck Surgery, Loudi Cenral Hospital, Hunan, China.
Asian J Surg. 2022 Dec;45(12):2651-2658. doi: 10.1016/j.asjsur.2022.01.075. Epub 2022 Mar 17.
CDH3 is recognized as an oncogene in various malignancies. Here, we aim to explore the association of CDH3 expression and prognostic implication in oral squamous cell carcinoma (OSCC).
Bioinformatics was used to analyze differentially expressed genes in the TCGA database. The OSCC tissues of 136 cases were used for immunohistochemistry. Cox proportional hazard analysis was used to analyze the relationship between prognostic factors, CDH3 expression and patient survival. Kaplan-Meier analysis was adopted to calculate survival rates. RT-qPCR and Western blot were performed to detect the expression levels of CDH3 in oral squamous cell lines. The cell viability and colony formation abilities were examined by CCK-8 and colony formation assays, respectively. Wound healing assay was performed to examine the invasion ability of cells.
CDH3 is up-regulated in oral squamous cell carcinoma and related to bad prognosis. Knock-down of CDH3 limited cell viability, colony formation ability, migration, invasion and chemoresistance of OSCC cells.
CDH3 is associated with a poor prognosis through promoting migration, invasion and chemoresistance in oral squamous cell carcinoma.
CDH3 被认为是多种恶性肿瘤中的癌基因。本研究旨在探讨 CDH3 表达与口腔鳞状细胞癌（OSCC）预后的关系。
采用生物信息学方法分析 TCGA 数据库中差异表达的基因。使用免疫组织化学方法检测 136 例 OSCC 组织中 CDH3 的表达。采用 Cox 比例风险分析探讨预后因素、CDH3 表达与患者生存的关系。采用 Kaplan-Meier 分析计算生存率。采用 RT-qPCR 和 Western blot 检测口腔鳞状细胞系中 CDH3 的表达水平。通过 CCK-8 和集落形成实验检测细胞活力和集落形成能力。通过划痕愈合实验检测细胞侵袭能力。
CDH3 在口腔鳞状细胞癌中上调，与不良预后相关。CDH3 敲低可限制 OSCC 细胞的活力、集落形成能力、迁移、侵袭和化疗耐药性。
CDH3 通过促进口腔鳞状细胞癌的迁移、侵袭和化疗耐药性与不良预后相关。
