Schwartz Z, Ornoy A, Soskolne W A
Diabetes Res. 1986 Jul;3(6):321-6.
The direct effects of insulin and glucose on bone modelling was studied in an in vitro system. 16 day old mice fetal radii and ulnae prelabelled with 45Ca were cultured for 48 hr in BGJ medium supplemented with either 4 mg/ml of human serum albumin or 10% fetal calf serum. Insulin at concentrations of 10(-8)-10(-10) M induced an increase in the periosteal bone formed as well as in calcium, phosphate and hydroxyproline content. Glucose at concentrations of 200-800 mg/dl decreased bone length, calcium, phosphate and hydroxyproline content. There was no change in the 45Ca released into the medium, by either insulin or glucose, implying that these substances have no effect on bone resorption. The results of this study imply that insulin and glucose have a direct effect on bone modelling and that they may be important factors in growth disturbances occurring during diabetic pregnancy.
在体外系统中研究了胰岛素和葡萄糖对骨塑形的直接影响。用45Ca预标记的16日龄小鼠胎儿桡骨和尺骨在补充有4mg/ml人血清白蛋白或10%胎牛血清的BGJ培养基中培养48小时。浓度为10(-8)-10(-10)M的胰岛素可使形成的骨膜骨以及钙、磷和羟脯氨酸含量增加。浓度为200-800mg/dl的葡萄糖可使骨长度、钙、磷和羟脯氨酸含量降低。胰岛素或葡萄糖释放到培养基中的45Ca没有变化，这意味着这些物质对骨吸收没有影响。本研究结果表明，胰岛素和葡萄糖对骨塑形有直接影响，它们可能是糖尿病妊娠期间发生生长紊乱的重要因素。
