Kadri Syeda Ulfath Tazeen, Mulla Sikandar I, Babu R Nagesh, Suchithra B, Bilal Muhammad, Ameen Fuad, Bharagava Ram Naresh, Saratale Ganesh Dattatraya, Ferreira Luiz Fernando Romanholo, Américo-Pinheiro Juliana Heloisa Pinê
Department of Biochemistry, School of Allied Health Sciences, REVA University, Bangalore 560064, India; Department of Biochemistry, Maharani's Science College for Women, Bangalore 560001, India.
Department of Biochemistry, School of Allied Health Sciences, REVA University, Bangalore 560064, India.
Int J Biol Macromol. 2022 May 1;206:768-776. doi: 10.1016/j.ijbiomac.2022.03.073. Epub 2022 Mar 17.
Calmodulin-binding transcription activator (CAMTA) are a group of transcription factors that are known to perform various important biological functions in plants. Here, we report 7 putative CAMTA transcription factors identified from finger millet transcriptome data. They were further analyzed for physicochemical properties, subcellular localization, conserved domains and motifs, Gene Ontology (GO) terms, phylogeny, 3D structure prediction and CAMTA-Ca-Calmodulin interaction through protein-protein docking. All EcCAMTAs were found to be localized in the nucleus and possessed a calmodulin binding domain (CaMBD). GO results indicated the involvement of CAMTAs in DNA binding and protein binding molecular functions. Phylogenetic analysis classified EcCAMTA genes into 3-subgroups. 3D-structure of CAMTA proteins was elucidated through ab-initio protein modeling and its interaction with Calmodulin was investigated by docking studies. Our study provides molecular insight into the structure and function of CAMTA genes in finger millet and also highlights the role of omics-based in-silico approaches for identification of novel gene families in the absence of a reference genome or annotated database. This being the first study of CAMTA transcription factor family in finger millet, it could serve as a resource for further studies of CAMTA genes either in finger millet or other related millets and cereal crops.
钙调蛋白结合转录激活因子（CAMTA）是一类转录因子，已知其在植物中发挥多种重要的生物学功能。在此，我们报告了从 finger millet 转录组数据中鉴定出的 7 个假定的 CAMTA 转录因子。对它们进行了进一步分析，包括理化性质、亚细胞定位、保守结构域和基序、基因本体（GO）术语、系统发育、三维结构预测以及通过蛋白质-蛋白质对接分析 CAMTA 与钙调蛋白的相互作用。所有 EcCAMTAs 均定位于细胞核，且具有钙调蛋白结合结构域（CaMBD）。GO 结果表明 CAMTAs 参与 DNA 结合和蛋白质结合分子功能。系统发育分析将 EcCAMTA 基因分为 3 个亚组。通过从头蛋白质建模阐明了 CAMTA 蛋白的三维结构，并通过对接研究研究了其与钙调蛋白的相互作用。我们的研究为 finger millet 中 CAMTA 基因的结构和功能提供了分子见解，同时也突出了基于组学的计算机模拟方法在缺乏参考基因组或注释数据库的情况下鉴定新基因家族的作用。这是 finger millet 中 CAMTA 转录因子家族的首次研究，可为 finger millet 或其他相关谷子和谷类作物中 CAMTA 基因的进一步研究提供资源。
