Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, 313000, PR China.
Basic Medical and Forensic Medicine, Baotou Medical College, Baotou, Inner Mongolia, 014060, PR China; Liaoning University of Traditional Chinese Medicine, Shenyang, 110847, PR China.
J Ethnopharmacol. 2022 Jun 28;292:115195. doi: 10.1016/j.jep.2022.115195. Epub 2022 Mar 16.
Sea buckthorn (Hippophae rhamnoides L.) is popularly used as a herbal medicine and food additive in the world. Total flavonoids of Hippophae rhamnoides (TFH) are reported to have anti-inflammatory and immunomodulatory activities.
The effects of TFH on atopic dermatitis (AD)-like lesions induced by MC903 in mice was elucidated in the study.
To induce AD-like lesions, MC903 was adopted to apply repeatedly on the left ear in C57BL/6 mice. After induction of AD-like lesions, 0.5% and 1% TFH cream was applied topically on ears of mice once a day for 8 days. The degree of skin lesions was evaluated by macroscopical and histological methods. Expressions of filaggrin (FLG) was evaluated by Western blotting. Real-time polymerase chain reaction (qPCR) was adopted to detect the mRNA expression of thymic stromal lymphopoietin (TSLP), interferon (IFN)-γ, interleukin (IL-4), tumor necrosis factor (TNF)-α in skin lesions. In vitro, Cytokine Antibody Arrays were performed to measure production of cytokines in IFN-γ/TNF-α-treated HaCaT cells, Western blotting was employed to detect the expressions of p-NF-κB, p-ERK and p-P38.
Topical application of TFH significantly improved the severity of dermatitis by inhibiting the infiltration of mast cell, increasing expression of FLG, decreasing the expressions of TNF-α, IL-4, IFN-γ and TSLP in skin lesions. TFH decreased the levels of IL-1α, IL-1β, IL-6, monocyte chemoattractant protein (MCP)-1, MCP-3, macrophage-derived chemokine (MDC), platelet-derived growth factor (PDGF)-BB, thymus and activation regulated chemokine (TARC) in the supernatants of the HaCaT cells treated by IFN-γ/TNF-α. Furthermore, expressions of p-NF-κB, p-ERK and p-P38 were also decreased by TFH administration with dose dependent manner in HaCaT cells treated by IFN-γ/TNF-α.
Topical application of TFH improved AD-like lesions in mice induced by MC903. Which exerted the effects of anti-inflammation and repairing skin barrier by regulating Th1/Th2 balance. This finding indicates that TFH is a novel potential agent for the external treatment of AD.
沙棘（Hippophae rhamnoides L.）作为草药和食品添加剂在世界范围内广泛使用。据报道，沙棘总黄酮（TFH）具有抗炎和免疫调节作用。
本研究阐明 TFH 对 MC903 诱导的特应性皮炎（AD）样病变的影响。
采用 MC903 重复涂抹于 C57BL/6 小鼠左耳诱导 AD 样病变，给予 0.5%和 1% TFH 乳膏，每天一次，共 8 天，涂于小鼠耳部。采用宏观和组织学方法评价皮肤病变程度。采用 Western blot 法评价丝聚合蛋白（FLG）的表达。采用实时聚合酶链反应（qPCR）检测皮肤病变中胸腺基质淋巴细胞生成素（TSLP）、干扰素（IFN）-γ、白细胞介素（IL）-4、肿瘤坏死因子（TNF）-α的 mRNA 表达。体外采用细胞因子抗体阵列检测 IFN-γ/TNF-α处理的 HaCaT 细胞产生的细胞因子，采用 Western blot 法检测 p-NF-κB、p-ERK 和 p-P38 的表达。
TFH 局部应用可显著改善皮炎严重程度，抑制肥大细胞浸润，增加皮肤病变中 FLG 的表达，降低 TNF-α、IL-4、IFN-γ和 TSLP 的表达。TFH 降低了 IFN-γ/TNF-α处理的 HaCaT 细胞上清液中白细胞介素 1α、白细胞介素 1β、白细胞介素 6、单核细胞趋化蛋白 1（MCP）-1、MCP-3、巨噬细胞来源趋化因子（MDC）、血小板衍生生长因子（PDGF）-BB、胸腺和激活调节趋化因子（TARC）的水平。此外，TFH 还可剂量依赖性地下调 IFN-γ/TNF-α处理的 HaCaT 细胞中 p-NF-κB、p-ERK 和 p-P38 的表达。
TFH 可改善 MC903 诱导的 AD 样病变小鼠的皮肤病变，通过调节 Th1/Th2 平衡发挥抗炎和修复皮肤屏障的作用。这一发现表明 TFH 是一种治疗 AD 的新型潜在外用药物。
