Hou Dian-Dong, Di Zheng-Hong, Qi Rui-Qun, Wang He-Xiao, Zheng Song, Hong Yu-Xiao, Guo Hao, Chen Hong-Duo, Gao Xing-Hua
Department of Dermatology, The First Hospital of China Medical University, and Key Laboratory of Dermatology, Ministry of Education and Public Health, Shenyang, China.
Skin Pharmacol Physiol. 2017;30(5):268-276. doi: 10.1159/000479528. Epub 2017 Sep 6.
The objective of this study was to evaluate the topical effects of sea buckthorn (SBT) oil on atopic dermatitis (AD)-like lesions in a mouse model generated by repeated topical administration of DNCB in BALB/c mice.
DNCB was applied repeatedly on the dorsal skin of mice to induce AD-like lesions. Following AD induction, SBT oil was applied daily on the dorsal skin for 4 weeks. The severity of skin lesions was examined macroscopically and histologically. We further measured the production of MDC/CCL22 and TARC/CCL17 in IFN-γ/TNF-α activated HaCaT cells.
Topically applied SBT oil in DNCB-treated mice ameliorated the severity score of dermatitis, decreased epidermal thickness, reduced spleen and lymph node weights, and prevented mast cell infiltration. In addition, SBT oil suppressed the Th2 chemokines TARC and MDC via dose-dependent inhibition of NF-κB, JAK2/STAT1, and p38-MAPK signaling pathways in IFN-γ/TNF-α-activated HaCaT cells.
These results suggest that SBT oil had a beneficial effect on AD-like skin lesions, partially via inhibition of the Th2 chemokines TARC and MDC in inflamed skin.
本研究的目的是评估沙棘（SBT）油对通过在BALB/c小鼠中反复局部应用二硝基氯苯（DNCB）所生成的小鼠特应性皮炎（AD）样病变的局部作用。
在小鼠背部皮肤反复应用DNCB以诱导AD样病变。诱导出AD后，每天在背部皮肤涂抹SBT油，持续4周。通过宏观和组织学检查皮肤病变的严重程度。我们还测量了在IFN-γ/TNF-α激活的HaCaT细胞中巨噬细胞衍生趋化因子（MDC）/CCL22和胸腺激活调节趋化因子（TARC）/CCL17的产生。
在经DNCB处理的小鼠中局部应用SBT油可改善皮炎严重程度评分，降低表皮厚度，减轻脾脏和淋巴结重量，并防止肥大细胞浸润。此外，SBT油通过在IFN-γ/TNF-α激活的HaCaT细胞中对NF-κB、JAK2/STAT1和p38丝裂原活化蛋白激酶（MAPK）信号通路的剂量依赖性抑制作用，抑制了Th2趋化因子TARC和MDC。
这些结果表明，SBT油对AD样皮肤病变具有有益作用，部分是通过抑制炎症皮肤中的Th2趋化因子TARC和MDC实现的。
