Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, PR China.
Basic Medical and Forensic Medicine, Baotou Medical college, Baotou, Inner Mongolia, PR China.
BMC Complement Med Ther. 2020 Aug 26;20(1):263. doi: 10.1186/s12906-020-02997-2.
Atopic dermatitis (AD) is a worldwide chronic skin disease which burden public health. Sea buckthorn (SBT) (Hippophae rhamnoides L., Elaeagnaceae) oil, as a traditional herbal medicine, has been used for disease treatment for many years. The effects of SBT oil on AD mouse model induced by repeated administration of 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice was evaluated in this study.
Mice were divided into four groups including the normal control group, AD model group, AD model group treated with SBT oil (5 ml/kg) and AD model group treated with SBT oil (10 ml/kg). Same volume at different concentrations of SBT oil was applied daily on the latter two groups by gavage for 15 days following AD model induction. The function of skin barrier and the production of IL-4, IFN-γ, TNF-α and TSLP were examined after animal sacrifice. The migration and mature of langerhans cell (LCs) in lymph node was further assessed by flow cytometry.
SBT oil alleviated dermatitis scores, decreased ear thickness, prevented infiltration of mast cell, reduced lymph node weight and depressed activity of Th2 cells. SBT oil also reduced the expression of IL-4, IFN-γ, TNF-α and TSLP in ear tissue, IgE level in serum and mRNA relative expression of IL-4, IFN-γ, TNF-α in lymph node. Moreover, SBT oil inhibited the migration of LCs cells from local lesions to lymph node and it's mature in lymph node.
These results suggest SBT oil had a beneficial effect either systemic or regional on DNCB-induced AD mice via maintain the balance of Th1/Th2 and may be a potential complementary candidate for AD treatment.
特应性皮炎（AD）是一种全球性的慢性皮肤疾病，给公共健康带来了负担。沙棘（Hippophae rhamnoides L.，胡颓子科）油作为一种传统草药，多年来一直用于疾病治疗。本研究评价了沙棘油对反复给予 2,4-二硝基氯苯（DNCB）诱导的 BALB/c 小鼠 AD 模型的影响。
将小鼠分为四组，包括正常对照组、AD 模型组、AD 模型组用沙棘油（5ml/kg）处理和 AD 模型组用沙棘油（10ml/kg）处理。在 AD 模型诱导后，后两组通过灌胃每天给予不同浓度的沙棘油，连续 15 天。处死动物后，检测皮肤屏障功能和白细胞介素 4（IL-4）、干扰素 γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）和胸腺基质淋巴细胞生成素（TSLP）的产生。通过流式细胞术进一步评估淋巴结中朗格汉斯细胞（LCs）的迁移和成熟。
沙棘油减轻了皮炎评分，降低了耳厚度，防止了肥大细胞浸润，减少了淋巴结重量，抑制了 Th2 细胞的活性。沙棘油还降低了耳部组织中 IL-4、IFN-γ、TNF-α和 TSLP 的表达、血清中 IgE 水平以及淋巴结中 IL-4、IFN-γ、TNF-α的 mRNA 相对表达。此外，沙棘油抑制了局部病变 LC 细胞向淋巴结的迁移及其在淋巴结中的成熟。
这些结果表明，沙棘油通过维持 Th1/Th2 的平衡，对 DNCB 诱导的 AD 小鼠具有系统或局部的有益作用，可能是 AD 治疗的一种有潜力的补充候选药物。
