多倍化影响属于单子叶嵌合荆豆凝集素（MCJ）家族的茉莉酸调节蛋白 Ta-JA1 的等位基因变异，而 Ta-JA1 存在于野生二粒小麦中。

Polyploidization affects the allelic variation of jasmonate-regulated protein Ta-JA1 belonging to the monocot chimeric jacalin (MCJ) family in wild emmer wheat.

机构信息

College of Agronomy, Sichuan Agricultural University, Chengdu, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China.

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.

出版信息

Gene. 2022 May 30;825:146399. doi: 10.1016/j.gene.2022.146399. Epub 2022 Mar 17.

DOI:10.1016/j.gene.2022.146399

PMID:35306115

Abstract

The jasmonate-regulated protein Ta-JA1 belongs to the monocot chimeric jacalin (MCJ) family and plays a vital role in stress resistance in wheat. However, the impact of wheat polyploidization on Ta-JA1 remains unclear. In this study, 149 members of the MCJ family were identified among members of Triticeae using a genome-wide approach. The genes were resolved into three clades; MCJ genes in each clade were derived from different donor genes during evolution. Segmental duplication may have been the primary driver, compared with tandem duplication, of expansion in the MCJ family of wheat. Gene loss and acquisition occurred during tetraploidization, and the core expansion of the family occurred after tetraploidization. Sequencing data for 2104 accessions of T. aestivum and 99 accessions of T. dicoccoides showed that Ta-JA1-2A and Ta-JA1 were highly conserved in common wheat, and four alleles (TdJA1-Ax2, TdJA1-Ay2, TdJA1-Ax3, and TdJA1-Ay3) were detected in T. dicoccoides. Using gene-specific markers, one AsJA1-B allele was detected in 11 Ae. speltoides accessions and one TuJA1-Ax1 allele was detected in 70 T. urartu accessions. Six alleles were detected on chromosome 2A: TdJA1-Ax1 (13 accessions), TdJA1-Ay1 (57 accessions), TdJA1-Ax2 (23 accessions), TdJA1-Ay2 (42 accessions), TdJA1-Ax3 (29 accessions), and TdJA1-Ay3 (251 accessions). Only one allele (TdJA1-B) on chromosome 2B was detected in 415 T. dicoccoides accessions. A geographical distribution analysis revealed that Israel hosted higher allelic variation than other regions. Quantitative reverse transcription PCR analysis indicated that divergence in expression has occurred among Ta-JA1 alleles and, notably, TdJA1-Ax1 and TdJA1-Ay1 showed significantly higher expression levels than the other four allelic types in T. dicoccoides. The present results contribute to an improved understanding of the effects of polyploidization on the MCJ gene family and the functions of Ta-JA1, and may be useful to enrich common wheat germplasm resources.

摘要

茉莉酸调节蛋白 Ta-JA1 属于单子叶嵌合 Jacalin（MCJ）家族，在小麦的抗逆性中发挥着重要作用。然而，小麦多倍体化对 Ta-JA1 的影响尚不清楚。本研究采用全基因组方法在三属植物中鉴定了 149 个 MCJ 家族成员。这些基因分为三个分支；每个分支中的 MCJ 基因都是在进化过程中由不同的供体基因衍生而来的。与串联复制相比，片段复制可能是小麦 MCJ 家族扩张的主要驱动力。在四倍体化过程中发生了基因丢失和获得，并且在四倍体化之后，该家族的核心得到了扩张。对 2104 份普通小麦和 99 份提莫菲维小麦的测序数据表明，Ta-JA1-2A 和 Ta-JA1 在普通小麦中高度保守，在提莫菲维小麦中检测到 4 个等位基因（TdJA1-Ax2、TdJA1-Ay2、TdJA1-Ax3 和 TdJA1-Ay3）。利用基因特异性标记，在 11 份节节麦中检测到一个 AsJA1-B 等位基因，在 70 份粗山羊草中检测到一个 TuJA1-Ax1 等位基因。在 2A 染色体上检测到 6 个等位基因：TdJA1-Ax1（13 个品系）、TdJA1-Ay1（57 个品系）、TdJA1-Ax2（23 个品系）、TdJA1-Ay2（42 个品系）、TdJA1-Ax3（29 个品系）和 TdJA1-Ay3（251 个品系）。在 415 份提莫菲维小麦中只检测到 2B 染色体上的一个等位基因（TdJA1-B）。地理分布分析表明，以色列的等位基因变异高于其他地区。定量反转录 PCR 分析表明，Ta-JA1 等位基因之间的表达已经出现分化，特别是在提莫菲维小麦中，TdJA1-Ax1 和 TdJA1-Ay1 的表达水平明显高于其他四种等位基因类型。本研究结果有助于更好地理解多倍体化对 MCJ 基因家族和 Ta-JA1 功能的影响，并可能有助于丰富普通小麦的种质资源。