Chen Yingshuang, Zhu Zeyu, Liu Xinyi, Jiang Yuliang, Shen Jian
Jiangsu Open Laboratory of Major Scientific Instrument and Equipment, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat, Sch Chem & Mat Sci, Nanjing Normal University, Nanjing 210023, Jiangsu, PR China.
Jiangsu Open Laboratory of Major Scientific Instrument and Equipment, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat, Sch Chem & Mat Sci, Nanjing Normal University, Nanjing 210023, Jiangsu, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jul 5;275:121141. doi: 10.1016/j.saa.2022.121141. Epub 2022 Mar 14.
Viscosity and hypochlorite (OCl) play an important role in biological activities and may cause negative effects at abnormal levels. In this study, a novel lysosome-target fluorescent probe BDHA was obtained based on a benzothiazole derivative. Probe BDHA showed linear ranges of detection for viscosity from 1.62 cP to 851.6 cP with a fluorescent turn-on response. It can also be used as a sensor for OCl with a turn-off response and showed a good linear range from 0 to 390 μM, with the detection limit calculated to be 2.8 μM. Moreover, BDHA can also be used to image viscosity and OCl levels in HeLa cells and zebrafish, owing to its excellent optical properties.
粘度和次氯酸盐（OCl）在生物活性中起重要作用，异常水平时可能会产生负面影响。在本研究中，基于苯并噻唑衍生物获得了一种新型的溶酶体靶向荧光探针BDHA。探针BDHA对粘度的检测线性范围为1.62厘泊至851.6厘泊，呈现荧光开启响应。它还可以用作OCl传感器，呈现荧光关闭响应，线性范围为0至390μM，计算得出的检测限为2.8μM。此外，由于其优异的光学性质，BDHA还可用于对HeLa细胞和斑马鱼中的粘度和OCl水平进行成像。
