Consejo Nacional de Ciencia y Tecnología Research Fellow, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Delegación Tlalpan, 14370 Ciudad de México, Mexico.
Laboratorio de Farmacología Conductual, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Col. San Lorenzo Huipulco, Delegación Tlalpan, 14370 Ciudad de México, Mexico.
Horm Behav. 2022 May;141:105154. doi: 10.1016/j.yhbeh.2022.105154. Epub 2022 Mar 17.
Estrogen replacement therapy (ERT) is an effective treatment for symptoms associated with climacteric and depression some women experience during perimenopause and menopause. The antidepressant-like effects of ERT may depend on the type of estrogen, age, and time when restitution is initiated after hormonal decline. Prolame is a synthetic steroid with estrogenic and antidepressant-like effects that may produce fewer adverse effects. We hypothesize that such actions of prolame on females depend on age and the duration of hormone deprivation period. We assessed the antidepressant-like effects of 17β-estradiol (E) and prolame in young and middle-aged rats across different post-ovariectomy (Ovx) time frames. Independent groups of young adults and middle-aged female rats were tested in the forced swimming test (FST) at 3, 8, 16, and 24 weeks post-Ovx. Prolame and E were administered in a sub-chronic schedule consisting of three injections before the FST. Likewise, the utero-trophic effects of these hormones were analyzed. We found that E and prolame reduced immobility in young rats 3 and 8 weeks after Ovx; in contrast, only prolame produced this effect in middle-aged rats three weeks post-Ovx. E and prolame increased the animals' utero-somatic index at all post-Ovx times, but the action of E and prolame produced a greater response in young adult rats. Our findings showed that the antidepressant-like effects of E and prolame depend on the post-Ovx time frame, age, and estrogen type. Interestingly, our results indicate that, in contrast to E, prolame maintained its antidepressant effect in middle-aged rats.
雌激素替代疗法（ERT）是治疗绝经期和围绝经期妇女相关症状和抑郁的有效方法。ERT 的抗抑郁作用可能取决于雌激素的类型、年龄以及激素下降后开始恢复的时间。普罗莱姆是一种具有雌激素和抗抑郁作用的合成类固醇，可能产生较少的不良反应。我们假设，普罗莱姆对女性的这种作用取决于年龄和激素剥夺期的持续时间。我们评估了 17β-雌二醇（E）和普罗莱姆对不同去卵巢（Ovx）后时间框架的年轻和中年大鼠的抗抑郁作用。在去卵巢后 3、8、16 和 24 周，分别对年轻成年和中年雌性大鼠进行了强迫游泳试验（FST）。普罗莱姆和 E 按照亚慢性方案给药，在 FST 前进行三次注射。同样，分析了这些激素的子宫营养作用。我们发现，E 和普罗莱姆降低了去卵巢后 3 和 8 周的年轻大鼠的不动性；相比之下，只有普罗莱姆在去卵巢后 3 周对中年大鼠产生了这种作用。E 和普罗莱姆增加了动物的子宫体指数，所有去卵巢后的时间点，但 E 和普罗莱姆的作用在年轻成年大鼠中产生了更大的反应。我们的研究结果表明，E 和普罗莱姆的抗抑郁作用取决于去卵巢后的时间框架、年龄和雌激素类型。有趣的是，我们的结果表明，与 E 不同，普罗莱姆在中年大鼠中保持了其抗抑郁作用。
