Lanzhou Institute of Food and Drug Inspection and Testing, Lanzhou, 730070, China.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, 730070, PR China.
Talanta. 2022 Jun 1;243:123385. doi: 10.1016/j.talanta.2022.123385. Epub 2022 Mar 14.
The analysis of toxic and harmful substances in food has attracted significant attention. In this work, a molecularly imprinted column coupled to gas chromatography-triple quadrupole tandem mass spectrometry method (MIC-GC-MS/MS) method for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in takeaway meal boxes was established by comparing the extraction results of 16 PAHs with 5 different sample pretreatment techniques, and the effects of food types and storage conditions on the migration of PAHs was studied by migration test. The conditions of extraction and chromatography-mass spectrometry were optimized. The results showed that the linear ranges were in the wide range of 1-100 ng mL for 16 PAHs, with the correlation coefficients (R) higher than 0.9983; the detection limits (LODs) and the limits of quantification (LOQs) were in the range of 0.08-0.42 μg kg and 0.24-1.26 μg kg , respectively; the spiked recoveries at three levels of 10, 20, 30 μg kg were in the range of 86.2%-107.7% with the relative standard deviation (RSD) less than 7.8%. Therefore, this method had high sensitivity and good reproducibility and was suitable for the determination of 16 PAHs in food contact materials. The migration test of PAHs showed that the migration amount of PAHs in takeaway meal boxes was closely related to food types, and the migration amount was positively correlated with contact temperature and contact time in a certain range.
食品中有毒有害物质的分析引起了人们的广泛关注。在这项工作中，通过比较 5 种不同样品预处理技术对 16 种多环芳烃（PAHs）的提取结果，建立了一种分子印迹柱-气相色谱-三重四极杆串联质谱法（MIC-GC-MS/MS），用于测定外卖餐盒中的 16 种多环芳烃。通过迁移试验研究了食品类型和储存条件对 PAHs 迁移的影响。优化了提取和色谱-质谱条件。结果表明，16 种 PAHs 的线性范围为 1-100ng mL，相关系数（R）均大于 0.9983；检测限（LOD）和定量限（LOQ）分别为 0.08-0.42μg kg 和 0.24-1.26μg kg；在 10、20、30μg kg 三个加标水平下的回收率为 86.2%-107.7%，相对标准偏差（RSD）小于 7.8%。因此，该方法灵敏度高，重现性好，适用于食品接触材料中 16 种多环芳烃的测定。PAHs 的迁移试验表明，外卖餐盒中 PAHs 的迁移量与食品类型密切相关，在一定范围内，迁移量与接触温度和接触时间呈正相关。
