Institute of Quality Standard and Testing Technology for Agro-Products, 74641Shandong Academy of Agricultural Sciences, Jinan, China.
Shandong Provincial Key Laboratory of Test Technology on Food Quality and Safety, Jinan, China.
Hum Exp Toxicol. 2022 Jan-Dec;41:9603271211072871. doi: 10.1177/09603271211072871.
Beta-carotene (β-carotene, BC) is one of the carotenoids most commonly consumed by humans. BCMO1 is expressed in various human tissues and is considered to be a key enzyme that converts BC into vitamin A. Studies indicated that BC-derived carotenoid signaling molecules affected the physiological functions of fat cells. In order to investigate the role and possible molecular mechanism of BC in mouse adipocytes, we conducted 4-group and 2-group difference analysis based on the data of GSE27271 chip in the Gene Expression Omnibus database. Genes differentially expressed in the inguinal white adipose tissue of mice were screened out and combined with the STRING database to construct protein-protein interaction (PPI) networks. Among them, Alb (albumin), Mug1 (murinoglobulin-1) and Uox (urate oxidase) genes were at relatively key positions and may affect the action of BC. Besides, Ppara (peroxisome proliferator-activated receptor alpha), Acly (ATP-citrate lyase) and Fabp5 (fatty acid-binding protein 5) genes constituted functional partners with many genes in the PPI network, and these genes may be Bcmo1 targeting molecules. Gene Ontology (GO) function and signaling pathways enrichment analysis were performed on the genes with protein interaction relationship in the PPI network. Fatty acid binding, cholesterol metabolic process, and regulation of fatty acid metabolic process were significantly enriched, and PPAR signaling pathway showed the most significant, indicating that BC and Bcmo1 might synergistically affect body metabolic functions such as fat metabolism. In general, BC and Bcmo1 may play a role in fat metabolism in mice, thereby affecting other functions or diseases.
β-胡萝卜素（β-carotene，BC）是人类最常食用的类胡萝卜素之一。BCMO1 在各种人体组织中表达，被认为是将 BC 转化为维生素 A 的关键酶。研究表明，BC 衍生的类胡萝卜素信号分子影响脂肪细胞的生理功能。为了研究 BC 在小鼠脂肪细胞中的作用和可能的分子机制，我们基于基因表达综合数据库中 GSE27271 芯片的数据进行了 4 组和 2 组差异分析。筛选出小鼠腹股沟白色脂肪组织中差异表达的基因，并与 STRING 数据库结合构建蛋白质-蛋白质相互作用（PPI）网络。其中，Alb（白蛋白）、Mug1（鼠球蛋白-1）和 Uox（尿酸氧化酶）基因处于相对关键位置，可能影响 BC 的作用。此外，Ppara（过氧化物酶体增殖物激活受体α）、Acly（三羧酸循环柠檬酸裂解酶）和 Fabp5（脂肪酸结合蛋白 5）基因与 PPI 网络中的许多基因构成功能伙伴，这些基因可能是 Bcmo1 的靶向分子。对 PPI 网络中具有蛋白质相互作用关系的基因进行基因本体论（GO）功能和信号通路富集分析。脂肪酸结合、胆固醇代谢过程和脂肪酸代谢过程的调节显著富集，PPAR 信号通路显示最显著，表明 BC 和 Bcmo1 可能协同影响脂肪代谢等身体代谢功能。总的来说，BC 和 Bcmo1 可能在小鼠的脂肪代谢中发挥作用，从而影响其他功能或疾病。
