Burger P, Letko G, Schulz H U, Müller W A, Heinrich P
Dtsch Z Verdau Stoffwechselkr. 1986;46(3):177-82.
The contribution of immunologic noxae to the damage of the cells and the induction of autodigestion in the course of acute pancreatitis in rats should be regarded. The isolation of intact acinar cells from rat pancreas offers a new approach to a more specific antiserum being directed against antigens at the outside of the cell membrane. Proof of circulating antibodies was performed using the indirect immunofluorescence reaction method (IFAR). A defined membrane fluorescence was observed at the surface of the acinar cells in vitro and a fluorescence at the septa of lobules marking the borders of acini in vivo.
应考虑免疫性有害物质在大鼠急性胰腺炎过程中对细胞损伤和自身消化诱导的作用。从大鼠胰腺分离完整的腺泡细胞为制备更特异的针对细胞膜外抗原的抗血清提供了一种新方法。使用间接免疫荧光反应法（IFAR）检测循环抗体。在体外观察到腺泡细胞表面有明确的膜荧光，在体内观察到标记腺泡边界的小叶间隔处有荧光。
