Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Paris, France; Sorbonne Université, Hôpital Pitié-Salpêtrière, Service de virologie, Paris, France.
Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Paris, France; Sorbonne Université, Hôpital Pitié-Salpêtrière, Service de virologie, Paris, France.
Clin Microbiol Infect. 2022 Jul;28(7):1027.e1-1027.e4. doi: 10.1016/j.cmi.2022.03.012. Epub 2022 Mar 17.
We aimed to characterize the evolution of humoral immune response up to 1 year after SARS-CoV-2 infection in healthcare workers (HCWs) during the first wave of COVID-19 in Paris.
Serum samples from 92 HCWs were tested at month 0 (M0), M6, and M12 after SARS-CoV-2 infection for IgG targeting the nucleocapsid (N), IgG targeting the receptor-binding domain (RBD) of spike (S) protein, IgA targeting S, and anti-RBD neutralizing antibodies. After M6, 46 HCWs received a single dose of COVID-19 vaccine.
We observed a significant decrease in all SARS-CoV-2 immunologic markers at M6 post-infection: median decreases were 0.26 log binding antibody units/mL (M0: 1.9 (interquartile range (IQR) 1.47-2.27); M6: 1.64 (IQR 1.22-1.92)) for anti-RBD IgG; 4.10 (index) (M0: 4.94 (IQR 2.72-6.82); M6: 0.84 (IQR 0.25-1.55)) for anti-N IgG; 0.64 (index) (M0: 2.50 (IQR 1.18-4.62); M6: 1.86 (IQR 0.85-3.54)) for anti-S IgA; and 24.4% (M0: 66.4 (IQR 39.7-82.5); M6: 42.0 (IQR 16.8-68.8)) inhibition activity for the RBD neutralizing antibodies. Between M6 and M12, anti-RBD IgG level, anti-S IgA index, and anti-RBD neutralizing activity significantly increased among COVID-19 vaccinated HCWs, whereas they remained stable among unvaccinated HCWs. Anti-N IgG index significantly decreased between M6 and M12 among both vaccinated (median: 0.73 (IQR 0.23-1.11) at M6 and 0.52 (IQR 0.20-0.73) at M12) and unvaccinated HCWs (median: 0.79 (IQR 0.21-4.67) at M6 and 0.34 (IQR 0.24-2.78) at M12).
A steady decline in the anti-N IgG response was observed during the first year after SARS-CoV-2 infection among HCWs, whereas the anti-RBD IgG and the anti-S IgA responses remained stable and could be enhanced by COVID-19 vaccination.
我们旨在描述在巴黎 COVID-19 第一波疫情期间，医护人员（HCWs）在感染 SARS-CoV-2 后 1 年内体液免疫反应的演变。
在感染 SARS-CoV-2 后第 0 个月（M0）、第 6 个月（M6）和第 12 个月（M12），对 92 名 HCWs 的血清样本进行检测，以检测针对核衣壳（N）的 IgG、针对刺突（S）蛋白受体结合域（RBD）的 IgG、针对 S 的 IgA 和抗 RBD 中和抗体。在 M6 之后，46 名 HCWs 接受了一剂 COVID-19 疫苗。
我们观察到感染后 6 个月时所有 SARS-CoV-2 免疫标志物均显著下降：抗 RBD IgG 的中位数下降了 0.26 个结合抗体单位/mL（M0：1.9（四分位距（IQR）1.47-2.27）；M6：1.64（IQR 1.22-1.92））；抗 N IgG 的中位数下降了 4.10（指数）（M0：4.94（IQR 2.72-6.82）；M6：0.84（IQR 0.25-1.55））；抗 S IgA 的中位数下降了 0.64（指数）（M0：2.50（IQR 1.18-4.62）；M6：1.86（IQR 0.85-3.54））；RBD 中和抗体的抑制活性下降了 24.4%（M0：66.4（IQR 39.7-82.5）；M6：42.0（IQR 16.8-68.8））。在 M6 和 M12 之间，COVID-19 疫苗接种的 HCWs 中抗 RBD IgG 水平、抗 S IgA 指数和抗 RBD 中和活性显著增加，而未接种疫苗的 HCWs 则保持稳定。在接种和未接种疫苗的 HCWs 中，抗 N IgG 指数均在 M6 和 M12 之间显著下降（M6 的中位数分别为 0.73（IQR 0.23-1.11）和 0.52（IQR 0.20-0.73）；M12 的中位数分别为 0.79（IQR 0.21-4.67）和 0.34（IQR 0.24-2.78））。
在感染 SARS-CoV-2 后的第一年，HCWs 中针对 N 的 IgG 反应持续下降，而针对 RBD IgG 和针对 S 的 IgA 反应保持稳定，并且可以通过 COVID-19 疫苗接种增强。
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