Dong Jun-Fang, Feng Cheng-Jie, Wang Ping, Li Ren-Qing, Zou Qing-Hua
Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China; Department of Clinical Laboratory, Tianjin University Children's Hospital, Tianjin, 300074, China.
Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, China.
Microb Pathog. 2022 Apr;165:105492. doi: 10.1016/j.micpath.2022.105492. Epub 2022 Mar 17.
The incidence of multidrug-resistant Acinetobacter baumannii has posed a major challenge for clinical treatment. There is still a significant gap in understanding the mechanism causing multi-drug resistance (MDR). In this study, the genomes of 10 drug sensitive and 10 multi-drug resistant A.baumannii strains isolated from a hospital in China were sequenced and compared. The antibiotic resistance genes, virulence factors were determined and CRIPSR-Cas system along with prophages were detected. The results showed that MDR strains are significantly different from the drug sensitive strains in the CARD entries, patterns of sequences matching up to plasmids, VFDB entries and CRISPR-Cas system. MDR strains contain unique CARD items related to antibiotic resistance which are absent in sensitive strains. Furthermore, sequences from genomes of MDR strains can match up with plasmids from more diversified bacteria genera compared to drug sensitive strains. MDR strains also contain a lower level of CRISPR genes and larger amount of prophages, along with higher levels of spacer sequences. These findings provide new experimental evidences for the study of the antibiotic resistance mechanism of A. baumannii.
多重耐药鲍曼不动杆菌的发生率给临床治疗带来了重大挑战。在理解导致多重耐药（MDR）的机制方面仍存在显著差距。在本研究中，对从中国一家医院分离出的10株药物敏感鲍曼不动杆菌菌株和10株多重耐药鲍曼不动杆菌菌株的基因组进行了测序和比较。确定了抗生素抗性基因、毒力因子，并检测了CRIPSR-Cas系统以及原噬菌体。结果表明，多重耐药菌株在CARD条目、与质粒匹配的序列模式、VFDB条目和CRIPSR-Cas系统方面与药物敏感菌株有显著差异。多重耐药菌株含有与抗生素抗性相关的独特CARD项目，而敏感菌株中不存在这些项目。此外，与药物敏感菌株相比，多重耐药菌株基因组的序列能与更多不同细菌属的质粒匹配。多重耐药菌株还含有较低水平的CRISPR基因和大量的原噬菌体，以及较高水平的间隔序列。这些发现为鲍曼不动杆菌抗生素抗性机制的研究提供了新的实验证据。
