Health and Animal Production-Veterinary Medicine, Unopar, Arapongas, PR, Brazil.
LabTronVet-Veterinary Medicine, Universidade Federal do Pará, Castanhal, PA, Brazil.
Trop Anim Health Prod. 2022 Mar 21;54(2):137. doi: 10.1007/s11250-022-03147-1.
The aim of the present study was to evaluate the efficiency of using in vitro fertilization to validate semen fertility for artificial insemination. Cryopreserved semen from ten bulls (five Nelore and five Brangus bulls) was evaluated using in vitro production of embryos (IVPE) and via fixed-time artificial insemination (FTAI). There was variation (p < 0.05) in the IVPE (20.9 to 53.7% of blastocyst production) and in the FTAI (42.0 to 56.0% of pregnant cows) results among the semen evaluated. According to the results, there was a positive correlation (rs = 0.8378; p = 0.0001) between the rate of blastocyst production (using IVPE) and the rate of pregnancy (using FTAI) using Nelore bull semen. Variation (p < 0.05) was also found using semen from Brangus bulls, in the rates of blastocyst production (36.5 to 47.0%) and pregnancy (45.6 to 52.2%) via FTAI. There was also a positive correlation (rs = 0.8786; p = 0.0001) between the rates of blastocyst production (IVPE) and pregnancy (FTAI) when using Brangus bull semen. According to the results, IVPE may be used in addition to conventional semen analysis to evaluate and validate the semen fertility of bulls for artificial insemination programs.
本研究旨在评估体外受精在验证人工授精精液生育力方面的效率。使用体外胚胎生产（IVPE）和定时人工授精（FTAI）评估了来自 10 头公牛（5 头 Nelore 公牛和 5 头 Brangus 公牛）的冷冻精液。IVPE（胚泡产生率为 20.9%至 53.7%）和 FTAI（受孕母牛率为 42.0%至 56.0%）的结果存在差异（p<0.05）。根据结果，使用 Nelore 公牛精液进行 IVPE 时，胚泡产生率（使用 IVPE）与 FTAI 时的受孕率（使用 FTAI）之间存在正相关（rs=0.8378；p=0.0001）。使用 Brangus 公牛精液进行 FTAI 时，胚泡产生率（36.5%至 47.0%）和受孕率（45.6%至 52.2%）也存在差异（p<0.05）。使用 Brangus 公牛精液进行 IVPE 时，胚泡产生率（IVPE）与 FTAI 时的受孕率（FTAI）之间也存在正相关（rs=0.8786；p=0.0001）。根据结果，IVPE 可用于常规精液分析之外，以评估和验证人工授精计划中公牛的精液生育力。
