Hasbi Hasbi, Iskandar Hikmayani, Sonjaya Herry, Purwantara Bambang, Arifiantini Raden Iis, Agil Muhammad, Pardede Berlin Pandapotan, Suyadi Suyadi, Septian Wike Andre, Samsudewa Daud, Damayanti Erni, Maulana Tulus, Said Syahruddin
Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia.
Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16914, Indonesia.
Vet World. 2024 Mar;17(3):593-601. doi: 10.14202/vetworld.2024.593-601. Epub 2024 Mar 17.
Fertility is crucial for enhancing the efficiency of livestock production, as it directly impacts the reproductive rates. A comprehensive understanding of the relationship between sperm quality and embryo development is key to optimizing reproductive outcomes and improving the quality of livestock. This study analyzed the developmental competence of embryos recovered from Bali cattle with normal or poor sperm motility.
Nine bulls with normal fresh semen (NFS) or poor fresh semen (PFS) motility were ejaculated for semen. Semen ejaculates, including volume, motility, and sperm concentration, were evaluated immediately after collection to measure the quality of the fresh semen. Frozen semen was evaluated using computer-assisted semen analysis (CASA) for motility, progressive sperm motility, distance curve path, distance curve linear, distance straight line, average path velocity, curvilinear velocity, linear velocity, straightness (STR), linearity of forward progression (LIN), wobble, and average lateral head displacement (ALH). Bull groups were used to determine embryo cleavage ability after fertilization of Bali cattle. Ovaries of Bali cattle were collected by slicing, and only cytoplasmic oocytes with compact cumulus cells were used in this study. The oocytes were matured, and fertilization was performed using fertilization media with a final sperm concentration of 1.5 × 10 spermatozoa/mL. After 48 h, the embryo cleavage ability of the cultured oocytes was evaluated.
There were significant differences in motility values between the NFS and PFS groups; however, there were no significant differences in the volume or sperm concentration. There was a significant difference in the LIN value between the groups but no significant differences in other CASA parameters. There were no significant differences in the cleavage rate and morula between the groups, but a positive correlation was observed between the cleavage rate and the morula and between the morula and ALH. A significant negative correlation was observed between the cleavage rate and STR and between the morula and STR; no significant differences were observed for other variables.
Despite variations in sperm characteristics, both normal and poor sperm motility demonstrated comparable embryonic development competence. These findings provide important insights into the fertility potential of Bali bulls, providing valuable information that can enhance selection strategies to improve the quality of livestock production.
繁殖力对于提高家畜生产效率至关重要，因为它直接影响繁殖率。全面了解精子质量与胚胎发育之间的关系是优化繁殖结果和提高家畜质量的关键。本研究分析了从巴厘岛牛采集的精子活力正常或低下的胚胎的发育能力。
对9头新鲜精液活力正常（NFS）或低下（PFS）的公牛进行采精。精液采集后立即评估射精量、活力和精子浓度，以测量新鲜精液的质量。使用计算机辅助精液分析（CASA）评估冷冻精液活力、精子前向运动活力、曲线轨迹距离、曲线轨迹线性度、直线距离、平均路径速度、曲线速度、直线速度（STR）前向运动线性度（LIN）、摆动和平均侧向头部位移（ALH）。使用公牛组来确定巴厘岛牛受精后的胚胎分裂能力。通过切片采集巴厘岛牛的卵巢，本研究仅使用具有紧密卵丘细胞的细胞质卵母细胞。卵母细胞成熟后，使用最终精子浓度为1.5×10精子/mL的受精培养基进行受精。48小时后，评估培养的卵母细胞的胚胎分裂能力。
NFS组和PFS组之间的活力值存在显著差异；然而，射精量或精子浓度没有显著差异。两组之间的LIN值存在显著差异，但其他CASA参数没有显著差异。两组之间的分裂率和桑椹胚率没有显著差异，但观察到分裂率与桑椹胚率之间以及桑椹胚率与ALH之间呈正相关。观察到分裂率与STR之间以及桑椹胚率与STR之间存在显著负相关；其他变量没有显著差异。
尽管精子特征存在差异，但精子活力正常和低下的情况均表现出相当的胚胎发育能力。这些发现为巴厘岛公牛的繁殖潜力提供了重要见解，提供了有价值的信息，可增强选择策略以提高家畜生产质量。
