Purohit Ayushi, Sharma Radheshyam, Shiv Ramakrishnan R, Sharma Stuti, Kumar Ashish, Jain Devendra, Kushwaha Himmat S, Maharjan Elina
Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur 482 004, India.
Maharana Pratap University of Agriculture and Technology, Udaipur 313001, India.
Bioinorg Chem Appl. 2022 Mar 10;2022:6825150. doi: 10.1155/2022/6825150. eCollection 2022.
Nanoparticles show the multidisciplinary versatile utility and are gaining the prime place in various fields, such as medicine, electronics, pharmaceuticals, electrical designing, cosmetics, food industries, and agriculture, due to their small size and large surface to volume ratio. Biogenic or green synthesis methods are environmentally friendly, economically feasible, rapid, free of organic solvents, and reliable over conventional methods. Plant extracts are of incredible potential in the biosynthesis of metal nanoparticles owing to their bountiful availability, stabilizing, and reducing ability. In the present study, the aqueous leaf extract of Spreng was mixed with 0.5 mM silver nitrate and incubated at 70°C for 1 h and synthesized a good quantity of AgNPs. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray diffractometry (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The maximum absorption of UV-visible spectra was obtained in the range of 420-430 nm. Furthermore, SEM and TEM results inferred that the size of the particles were 23-62 nm, spherical, crystalline, uniformly distributed, and negatively charged with the zeta potential of -27.6 mV. In addition, the antifungal activities of the AgNPs were evaluated against two phytopathogenic fungi and f. sp. in vitro using poison food techniques on PDA media. The maximum rate of mycelia inhibition was found in 150 ppm concentration of AgNPs against both phytopathogenic fungi.
纳米粒子具有多学科的通用效用，由于其尺寸小和表面积与体积比大，在医学、电子、制药、电气设计、化妆品、食品工业和农业等各个领域正占据重要地位。生物合成或绿色合成方法对环境友好、经济可行、快速、无有机溶剂，且比传统方法更可靠。植物提取物因其丰富的可用性、稳定和还原能力，在金属纳米粒子的生物合成中具有巨大潜力。在本研究中，将Spreng的水叶提取物与0.5 mM硝酸银混合，在70°C下孵育1小时，合成了大量的AgNPs。使用紫外可见光谱、X射线衍射（XRD）、动态光散射（DLS）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对合成的AgNPs进行了表征。紫外可见光谱的最大吸收在420-430 nm范围内。此外，SEM和TEM结果表明，颗粒尺寸为23-62 nm，呈球形、结晶状、均匀分布，带负电荷，zeta电位为-27.6 mV。此外，使用PDA培养基上的毒饵技术，在体外评估了AgNPs对两种植物病原真菌和f. sp.的抗真菌活性。在150 ppm浓度的AgNPs对两种植物病原真菌中均发现了最大的菌丝抑制率。
