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一种来自沃克氏菌的具有抗乙肝病毒活性的多糖及…… （原文似乎不完整）

A Polysaccharide From Walker With Anti-HBV Activities and .

作者信息

Zhang Xue, Su Huiling, Yu Haifei, Ding Jialu, Deng Wanyu, Qin Bo, Zhou Changlin, Dou Jie, Guo Min

机构信息

State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

College of Life Science, Shangrao Normal University, Shangrao, China.

出版信息

Front Pharmacol. 2022 Mar 3;13:827128. doi: 10.3389/fphar.2022.827128. eCollection 2022.

DOI:10.3389/fphar.2022.827128
PMID:35308231
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8928433/
Abstract

Hepatitis B virus (HBV) infection remains a major global threat to human health worldwide. Recently, the Chinese medicines with antiviral properties and low toxicity have been a concern. In our previous study, Walker polysaccharide (ESPS) has been isolated and characterized, while its antiviral effect on HBV remained unclear. The anti-HBV activity of ESPS and its regulatory pathway were investigated and . The results showed that ESPS significantly inhibited the production of HBsAg, HBeAg, and HBV DNA in the supernatants of HepG2.2.15 in a dose-dependent manner; HBV RNA and core protein expression were also decreased by ESPS. The studies using HBV transgenic mice further revealed that ESPS (20 and 40 mg/kg/2 days) significantly reduced the levels HBsAg, HBeAg, and HBV DNA in the serum, as well as HBV DNA and HBV RNA in mice liver. In addition, ESPS activated the Toll-like receptor 4 (TLR4) pathway; elevated levels of IFN-β, TNF-α, and IL-6 in the serum were observed, indicating that the anti-HBV effect of ESPS was achieved by potentiating innate immunity function. In conclusion, our study shows that ESPS is a potential anti-HBV ingredient and is of great value in the development of new anti-HBV drugs.

摘要

乙型肝炎病毒（HBV）感染仍然是全球人类健康面临的主要威胁。最近，具有抗病毒特性且低毒的中药受到关注。在我们之前的研究中，已分离并鉴定出云芝多糖（ESPS），但其对HBV的抗病毒作用仍不清楚。研究了ESPS的抗HBV活性及其调控途径。结果表明，ESPS以剂量依赖的方式显著抑制HepG2.2.15上清液中HBsAg、HBeAg和HBV DNA的产生；ESPS还降低了HBV RNA和核心蛋白的表达。使用HBV转基因小鼠的研究进一步表明，ESPS（20和40mg/kg/2天）显著降低了血清中HBsAg、HBeAg和HBV DNA的水平，以及小鼠肝脏中HBV DNA和HBV RNA的水平。此外，ESPS激活了Toll样受体4（TLR4）途径；观察到血清中IFN-β、TNF-α和IL-6水平升高，表明ESPS的抗HBV作用是通过增强固有免疫功能实现的。总之，我们的研究表明ESPS是一种潜在的抗HBV成分，在开发新型抗HBV药物方面具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/bc7f5b37191c/fphar-13-827128-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/dc3bf5b7c295/fphar-13-827128-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/2e61f9a88b48/fphar-13-827128-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/ea5bb75e031e/fphar-13-827128-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/73e96bc2a61a/fphar-13-827128-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/473e9413108a/fphar-13-827128-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/bf8001738bdb/fphar-13-827128-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/bc7f5b37191c/fphar-13-827128-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/dc3bf5b7c295/fphar-13-827128-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/2e61f9a88b48/fphar-13-827128-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/ea5bb75e031e/fphar-13-827128-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/73e96bc2a61a/fphar-13-827128-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/473e9413108a/fphar-13-827128-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/bf8001738bdb/fphar-13-827128-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a8f/8928433/bc7f5b37191c/fphar-13-827128-g007.jpg

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