Pĕknicová J, Capková J, Cechová D, Sulcová B
Folia Biol (Praha). 1986;32(4):282-5.
BALB/c mice were immunized with the crude fraction of boar acrosin. Immune spleen cells were fused with myeloma cells SP2/0-Ag14. One of the 6 hybridomas produced was cloned and characterized by ELISA and SDS-PAGE. Possible uses of the monoclonal antibody against acrosin for immunological detection of the amount of acrosin liberated after manipulations with spermatozoa and for selection of undamaged spermatozoa for insemination are discussed.
用猪顶体蛋白酶粗提物免疫BALB/c小鼠。将免疫后的脾细胞与骨髓瘤细胞SP2/0-Ag14进行融合。对产生的6个杂交瘤中的1个进行克隆，并通过酶联免疫吸附测定法（ELISA）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行鉴定。讨论了抗顶体蛋白酶单克隆抗体在免疫检测精子操作后释放的顶体蛋白酶量以及选择未受损精子用于授精方面的可能用途。
