Saglik Imran, Turkkan Alparslan, Turan Cansu, Kara Ates, Akalin Halis, Ener Beyza, Sahin Ahmet, Yesil Edanur, Celebi Solmaz, Kazak Esra, Heper Yasemin, Yilmaz Emel, Korkmaz Muhammet Furkan, Ture Esra, Hacimustafaoglu Mustafa
Department of Medical Microbiology, Bursa Uludag University Medical Faculty, Bursa, TUR.
Department of Public Health Sciences, Bursa Uludag University Medical Faculty, Bursa, TUR.
Cureus. 2022 Feb 14;14(2):e22195. doi: 10.7759/cureus.22195. eCollection 2022 Feb.
Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific humoral immune persistence has been proposed to be affected by patients' characteristics. Moreover, available conflicting assay results are needed to be settled through comparative research with defined clinical specimens. Methods This prospective study investigated SARS-CoV-2-specific antibodies among 43 adults and 34 children at a mean of 12 weeks after the onset of COVID-19 symptoms using six serological assays and compared their performance. We used two Euroimmun (Euroimmun, Luebeck, Germany), two automated Roche Elecsys (Basel, Switzerland), and two rapid immuno-chromatographic Ecotest (Matrix Diagnostics, Assure Tech. (Hangzhou) Co., L, China) assays to investigate SARS-CoV-2 antibodies. Results The findings showed that the Roche Elecsys anti-S total test yielded the best positivity/sensitivity (children 94.1% and adults 93.0%; p = 0.877) while five immunoglobulin IgG targeting assays had similar positivity/sensitivity between children (88.2% to 94.1%) and adults (88.4% to 93.0%) (p > 0.05). Although IgM positivity was relatively low (p < 0.001), it was found in the majority of our pediatric and adult patients (67.6% and 86.0%, respectively; p = 0.098). SARS-CoV-2 S IgG titers were found to be higher among males in pediatric and adult groups compared to females (p = 0.027 and p = 0.041, respectively). Furthermore, we observed significantly higher antibody titers among pneumonia patients (p = 0.001). Conclusion Overall, we concluded SARS-CoV-2 antibody persistence over an average of 12 weeks after the onset of COVID-19 symptoms. While automated Roche Elecsys total antibody assays yielded the best sensitivity (> 90%) and five assays targeting IgG had acceptable performance. Patients with pneumonia and males have higher antibody titers. The effect of antibody persistence on re-infections should be monitored in longitudinal studies.
引言 严重急性呼吸综合征冠状病毒2（SARS-CoV-2）特异性体液免疫持久性被认为受患者特征影响。此外，现有的相互矛盾的检测结果需要通过与明确的临床标本进行比较研究来解决。方法 这项前瞻性研究使用六种血清学检测方法，对43名成人和34名儿童在出现新冠病毒病（COVID-19）症状后平均12周时的SARS-CoV-2特异性抗体进行了调查，并比较了它们的性能。我们使用两种欧蒙（Euroimmun，德国吕贝克）、两种罗氏全自动电化学发光免疫分析系统（Roche Elecsys，瑞士巴塞尔）以及两种快速免疫层析法爱康特（Ecotest，美创诊断技术（杭州）有限公司，中国）检测方法来检测SARS-CoV-2抗体。结果 研究结果显示，罗氏电化学发光免疫分析系统抗S总抗体检测的阳性率/敏感性最佳（儿童为94.1%，成人为93.0%；p = 0.877），而五种针对免疫球蛋白IgG的检测方法在儿童（88.2%至94.1%）和成人间的阳性率/敏感性相似（88.4%至93.0%）（p > 0.05）。虽然IgM阳性率相对较低（p < 0.001），但在我们大多数儿科和成年患者中均检测到（分别为67.6%和86.0%；p = 0.098）。在儿科和成年组中，男性的SARS-CoV-2 S IgG滴度均高于女性（分别为p = 0.027和p = 0.041）。此外，我们观察到肺炎患者的抗体滴度明显更高（p = 0.001）。结论 总体而言，我们得出结论，在出现COVID-19症状后平均12周内SARS-CoV-2抗体持续存在。罗氏全自动电化学发光免疫分析系统总抗体检测的敏感性最佳（> 90%），五种针对IgG的检测方法性能也可接受。肺炎患者和男性的抗体滴度更高。抗体持久性对再次感染的影响应在纵向研究中进行监测。
