Syanda Adam M, Kringstad Vera I, Blackford Samuel J I, Kjesbu Joachim S, Ng Soon Seng, Ma Liang, Xiao Fang, Coron Abba E, Rokstad Anne Mari A, Modi Sunil, Rashid S Tamir, Strand Berit Løkensgard
Department of Metabolism, Digestion and Reproduction, Imperial College London (ICL), London, United Kingdom.
Department of Biotechnology and Food Science, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
Front Bioeng Biotechnol. 2022 Mar 3;9:816542. doi: 10.3389/fbioe.2021.816542. eCollection 2021.
Intra-peritoneal placement of alginate encapsulated human induced pluripotent stem cell-derived hepatocytes (hPSC-Heps) represents a potential new bridging therapy for acute liver failure. One of the rate-limiting steps that needs to be overcome to make such a procedure more efficacious and safer is to reduce the accumulation of fibrotic tissue around the encapsulated cells to allow the free passage of relevant molecules in and out for metabolism. Novel chemical compositions of alginate afford the possibility of achieving this aim. We accordingly used sulfated alginate and demonstrated that this material reduced fibrotic overgrowth whilst not impeding the process of encapsulation nor cell function. Cumulatively, this suggests sulfated alginate could be a more suitable material to encapsulate hPSC-hepatocyte prior to human use.
腹腔内植入藻酸盐包封的人诱导多能干细胞衍生的肝细胞（hPSC-Heps）代表了一种治疗急性肝衰竭的潜在新的桥接疗法。为使该程序更有效、更安全而需要克服的限速步骤之一是减少包封细胞周围纤维化组织的积累，以允许相关分子自由进出进行代谢。藻酸盐的新型化学成分提供了实现这一目标的可能性。因此，我们使用了硫酸化藻酸盐，并证明这种材料减少了纤维化过度生长，同时不阻碍包封过程和细胞功能。总的来说，这表明硫酸化藻酸盐可能是一种更适合在人体使用前包封hPSC-肝细胞的材料。
